Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

NF-kB p65/RelA Rabbit pAb (A11202)

Reviews (5) Publications (4) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - NF-kB p65/RelA Rabbit pAb (A11202)

Western blot analysis of extracts of various cell lines, using NF-kB p65/RelA antibody (A11202) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - NF-kB p65/RelA Rabbit pAb (A11202)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using NF-kB p65/RelA Rabbit pAb (A11202) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A11202)

Immunofluorescence analysis of NIH/3T3 cells using NF-kB p65/RelA Rabbit pAb (A11202) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for NF-kB p65/RelA Rabbit pAb(A11202)
Review
ABclonal: review for NF-kB p65/RelA Rabbit pAb(A11202)
Review
ABclonal: review for NF-kB p65/RelA Rabbit pAb(A11202)
Review
ABclonal: review for NF-kB p65/RelA Rabbit pAb(A11202)
Review
ABclonal: review for NF-kB p65/RelA Rabbit pAb(A11202)

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Overview

Product name NF-kB p65/RelA Rabbit pAb
Catalog No. A11202
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

NF-kappa-B is a ubiquitous transcription factor involved in several biological processes. It is held in the cytoplasm in an inactive state by specific inhibitors. Upon degradation of the inhibitor, NF-kappa-B moves to the nucleus and activates transcription of specific genes. NF-kappa-B is composed of NFKB1 or NFKB2 bound to either REL, RELA, or RELB. The most abundant form of NF-kappa-B is NFKB1 complexed with the product of this gene, RELA. Four transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 450-551 of human NF-kB p65/RelA (NP_068810.3).
Sequence LGALLGNSTDPAVFTDLASVDNSEFQQLLNQGIPVAPHTTEPMLMEYPEAITRLVTGAQRPPDPAPAPLGAPGLPNGLLSGDEDFSSIADMDFSALLSQISS
Gene ID 5970
Swiss prot Q04206
Synonyms p65; CMCU; NFKB3; AIF3BL3; NF-kB p65/RelA
Calculated MW 60kDa
Observed MW 65kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples NIH/3T3, PC-12
Cellular location Cytoplasm, Nucleus
Customer validation

WB (Mouse embryonal fibroblast cell, human cells, Human colon cancer, Rat cellsHomo sapiens, Rattus norvegicus)

IHC (Mouse embryonal fibroblast cellRattus norvegicus, Rattus norvegicus)

IP (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

NF-kB p65/RelA Rabbit pAb images

ABclonal:Western blot - NF-kB p65/RelA Rabbit pAb (A11202)}

Western blot - NF-kB p65/RelA Rabbit pAb (A11202)

Western blot analysis of extracts of various cell lines, using NF-kB p65/RelA antibody (A11202) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - NF-kB p65/RelA Rabbit pAb (A11202)}

Immunohistochemistry - NF-kB p65/RelA Rabbit pAb (A11202)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using NF-kB p65/RelA Rabbit pAb (A11202) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A11202)}

Immunofluorescence - NF-kB p65/RelA Rabbit pAb (A11202)

Immunofluorescence analysis of NIH/3T3 cells using NF-kB p65/RelA Rabbit pAb (A11202) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Reviews List

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Related Targets

Based on existing publications, the following genes are closely related to research on RELA. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to RELA. (Distance between topics and target gene indicate popularity.) RELA

* Data provided by citexs.com, for reference only.

Publishing research using A11202? Please let us know so that we can cite the reference in this datasheet.

Antibodies (22)

ELISA Kits (1)

Proteins (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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