Product Type > Antibodies > Primary Antibodies > Phospho-specific Antibodies

Phospho-ELK1-S383 Rabbit pAb (AP0033)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - Phospho-ELK1-S383 Rabbit pAb (AP0033)

Western blot analysis of extracts of Jurkat cells, using Phospho-ELK1-S383 antibody (AP0033) at 1:1000 dilution. Jurkat cells were treated by Anisomycin (5μg/mL) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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Overview

Product name Phospho-ELK1-S383 Rabbit pAb
Catalog No. AP0033
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum response element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. This gene produces multiple isoforms by using alternative translational start codons and by alternative splicing. Related pseudogenes have been identified on chromosomes 7 and 14.

Immunogen information

Immunogen A synthetic phosphorylated peptide around S383 of human ELK1 (NP_005220.2).
Sequence TLSPI
Gene ID 2002
Swiss prot P19419
Synonyms ELK1; Phospho-ELK1-S383
Calculated MW 45kDa
Observed MW 45kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Jurkat
Cellular location Nucleus

Research Area

Phospho-ELK1-S383 Rabbit pAb images

ABclonal:Western blot - Phospho-ELK1-S383 Rabbit pAb (AP0033)}

Western blot - Phospho-ELK1-S383 Rabbit pAb (AP0033)

Western blot analysis of extracts of Jurkat cells, using Phospho-ELK1-S383 antibody (AP0033) at 1:1000 dilution. Jurkat cells were treated by Anisomycin (5μg/mL) for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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Related Targets

Based on existing publications, the following genes are closely related to research on ELK1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ELK1. (Distance between topics and target gene indicate popularity.) ELK1

* Data provided by citexs.com, for reference only.

Publishing research using AP0033? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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