Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

PARP1 Rabbit pAb (A0942)

Reviews (3) Publications (42) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - PARP1 Rabbit pAb (A0942)

Western blot analysis of various lysates using PARP1 Rabbit pAb (A0942) at 1:3000 dilution.HeLa cells were treated by staurosporine(1 uM) for 3 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - PARP1 Rabbit pAb (A0942)

Western blot analysis of various lysates using PARP1 Rabbit pAb (A0942) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunofluorescence - PARP1 Rabbit pAb (A0942)

Confocal immunofluorescence analysis of U-2 OS cells using PARP1 Rabbit pAb (A0942) at dilution of 1:200. Blue: DAPI for nuclear staining.

Review
ABclonal: review for PARP1 Rabbit pAb(A0942)
Review
ABclonal: review for PARP1 Rabbit pAb(A0942)
Review
ABclonal: review for PARP1 Rabbit pAb(A0942)

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Overview

Product name PARP1 Rabbit pAb
Catalog No. A0942
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 81-390 of human PARP1 (NP_001609.2).
Sequence DQQKVKKTAEAGGVTGKGQDGIGSKAEKTLGDFAAEYAKSNRSTCKGCMEKIEKGQVRLSKKMVDPEKPQLGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLPGVKSEGKRKGDEVDGVDEVAKKKSKKEKDKDSKLEKALKAQNDLIWNIKDELKKVCSTNDLKELLIFNKQQVPSGESAILDRVADGMVFGALLPCEECSGQLVFKSDAYYCTGDVTAWTKCMVKTQTPNRKEWVTPKEFREISYLKKLKVKKQDRIFPPETSASVAATPPPSTASAPAAVNSSASADKPL
Gene ID 142
Swiss prot P09874
Synonyms PARP; PARS; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1; Poly-PARP; PARP1
Calculated MW 113kDa
Observed MW 89kDa/113kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HeLa, Jurkat, A-549, Mouse testis, Rat testis
Cellular location Nucleus, nucleolus
Customer validation

WB (NCI-N87 cells, Eoma cell, HCC827Homo sapiens, Homo sapiens, Bos taurus, Mus musculus, Giardia duodenalis, Rattus norvegicus, H. cordata)

IF (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

PARP1 Rabbit pAb images

ABclonal:Western blot - PARP1 Rabbit pAb (A0942)}

Western blot - PARP1 Rabbit pAb (A0942)

Western blot analysis of various lysates using PARP1 Rabbit pAb (A0942) at 1:3000 dilution.HeLa cells were treated by staurosporine(1 uM) for 3 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - PARP1 Rabbit pAb (A0942)}

Western blot - PARP1 Rabbit pAb (A0942)

Western blot analysis of various lysates using PARP1 Rabbit pAb (A0942) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunofluorescence - PARP1 Rabbit pAb (A0942)}

Immunofluorescence - PARP1 Rabbit pAb (A0942)

Confocal immunofluorescence analysis of U-2 OS cells using PARP1 Rabbit pAb (A0942) at dilution of 1:200. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on PARP1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to PARP1. (Distance between topics and target gene indicate popularity.) PARP1

* Data provided by citexs.com, for reference only.

Publishing research using A0942? Please let us know so that we can cite the reference in this datasheet.

Antibodies (7)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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