Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

KLF4 Rabbit pAb (A11853)

Publication (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - KLF4 Rabbit pAb (A11853)

Western blot analysis of various lysates using KLF4 Rabbit pAb (A11853) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Overview

Product name KLF4 Rabbit pAb
Catalog No. A11853
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. Mice lacking this gene have a normal appearance but lose weight rapidly, and die shortly after birth due to fluid evaporation resulting from compromised epidermal barrier function. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen information

Immunogen A synthetic peptide of human KLF4
Sequence Email for sequence
Gene ID 9314
Swiss prot O43474
Synonyms EZF; GKLF; KLF4
Calculated MW 55kDa
Observed MW 63kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    
Positive samples HeLa, MCF7, SW480, A-431
Cellular location Nucleus
Customer validation

WB (Mus musculus, Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

KLF4 Rabbit pAb images

ABclonal:Western blot - KLF4 Rabbit pAb (A11853)}

Western blot - KLF4 Rabbit pAb (A11853)

Western blot analysis of various lysates using KLF4 Rabbit pAb (A11853) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on KLF4. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to KLF4. (Distance between topics and target gene indicate popularity.) KLF4

* Data provided by citexs.com, for reference only.

Publishing research using A11853? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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