Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

HIF1α Rabbit pAb (A11945)

Review (1) Publications (31) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - HIF1α Rabbit pAb (A11945)

Western blot analysis of lysates from HeLa cells, using HIF1α Rabbit pAb (A11945) at 1:1000 dilution.Hela cells were treated by Cobalt chloride (0.1 mM) at 37℃ for 4 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Review
ABclonal: review for HIF1α Rabbit pAb(A11945)

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Overview

Product name HIF1α Rabbit pAb
Catalog No. A11945
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 670-826 of HIF1α (NP_001521.1).
Sequence NRAGKGVIEQTEKSHPRSPNVLSVALSQRTTVPEEELNPKILALQNAQRKRKMEHDGSLFQAVGIGTLLQQPDDHAATTSLSWKRVKGCKSSEQNGMEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEVNAPIQGSRNLLQGEELLRALDQVN
Gene ID 3091
Swiss prot Q16665
Synonyms HIF1; MOP1; PASD8; HIF-1A; bHLHe78; HIF-1alpha; HIF1-ALPHA; HIF-1-alpha; HIF1α
Calculated MW 93kDa
Observed MW 120kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa, NIH/3T3, Rat kidney
Cellular location Cytoplasm, Nucleus, Nucleus speckle
Customer validation

WB (N9 cellsHomo sapiens, Gallus gallus, Homo sapiens, Rattus norvegicus, Mus musculus)

IF (Homo sapiens)

Co-IP (Homo sapiens)

IHC (Gallus gallus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

HIF1α Rabbit pAb images

ABclonal:Western blot - HIF1α Rabbit pAb (A11945)}

Western blot - HIF1α Rabbit pAb (A11945)

Western blot analysis of lysates from HeLa cells, using HIF1α Rabbit pAb (A11945) at 1:1000 dilution.Hela cells were treated by Cobalt chloride (0.1 mM) at 37℃ for 4 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

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Related Targets

Based on existing publications, the following genes are closely related to research on HIF1A. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to HIF1A. (Distance between topics and target gene indicate popularity.) HIF1A

* Data provided by citexs.com, for reference only.

Publishing research using A11945? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Proteins (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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