Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

GATA3 Mouse mAb (A5711)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - GATA3 Mouse mAb (A5711)

Western blot analysis of extracts of various cell lines, using GATA3 antibody (A5711).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name GATA3 Mouse mAb
Catalog No. A5711
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia.

Immunogen information

Immunogen A synthetic peptide of human GATA3
Sequence Email for sequence
Gene ID 2625
Swiss prot P23771
Synonyms HDR; HDRS; GATA3
Calculated MW 48kDa
Observed MW 48kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:20 - 1:50
  • FC 1:20 - 1:50
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    Immunofluorescence    Flow Cytometry    
Positive samples HeLa, MCF7
Cellular location Nucleus

Research Area

GATA3 Mouse mAb images

ABclonal:Western blot - GATA3 Mouse mAb (A5711)}

Western blot - GATA3 Mouse mAb (A5711)

Western blot analysis of extracts of various cell lines, using GATA3 antibody (A5711).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on GATA3. (Numbers indicate number of published articles where both targets appear.)

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Related Research Area

Based on existing publications, the following research topics are related to GATA3. (Distance between topics and target gene indicate popularity.) GATA3

* Data provided by citexs.com, for reference only.

Publishing research using A5711? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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