Products > Catalog Antibodies > Polyclonal Antibodies

DNMT3A Rabbit pAb (A3169)

Publications (3) Datasheet

ABclonal:Western blot - DNMT3A Polyclonal Antibody (A3169)

Western blot analysis of extracts of various cell lines, using DNMT3A antibody (A3169).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - DNMT3A Polyclonal Antibody (A3169)

Immunohistochemistry of paraffin-embedded rat brain using DNMT3A Antibody (A3169) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - DNMT3A Polyclonal Antibody (A3169)

Immunohistochemistry of paraffin-embedded human kidney using DNMT3A Antibody (A3169) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - DNMT3A Polyclonal Antibody (A3169)

Immunohistochemistry of paraffin-embedded mouse heart using DNMT3A Antibody (A3169) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - DNMT3A Polyclonal Antibody (A3169)

Immunofluorescence analysis of A549 cells using DNMT3A antibody (A3169).

Overview

Product nameDNMT3A Rabbit pAb
Catalog No.A3169
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.
ImmunogenRecombinant protein of human DNMT3A
SequenceEmail for sequence
Gene ID1788
Swiss protQ9Y6K1
SynonymsDNMT3A; DNMT3A2; M.HsaIIIA; TBRS; DNMT3A
Calculated MW16kDa/81kDa/101kDa
Observed MW95kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse spleen, Mouse skeletal muscle
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Mus musculus, Homo sapiens)

IF(Mus musculus)

IHC(Homo sapiens,Mus musculus)

ABclonal:Western blot - DNMT3A Polyclonal Antibody (A3169) }

Western blot - DNMT3A Polyclonal Antibody (A3169)

Western blot analysis of extracts of various cell lines, using DNMT3A antibody (A3169).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - DNMT3A Polyclonal Antibody (A3169) }

Immunohistochemistry - DNMT3A Polyclonal Antibody (A3169)

Immunohistochemistry of paraffin-embedded rat brain using DNMT3A Antibody (A3169) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - DNMT3A Polyclonal Antibody (A3169) }

Immunohistochemistry - DNMT3A Polyclonal Antibody (A3169)

Immunohistochemistry of paraffin-embedded human kidney using DNMT3A Antibody (A3169) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - DNMT3A Polyclonal Antibody (A3169) }

Immunohistochemistry - DNMT3A Polyclonal Antibody (A3169)

Immunohistochemistry of paraffin-embedded mouse heart using DNMT3A Antibody (A3169) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - DNMT3A Polyclonal Antibody (A3169) }

Immunofluorescence - DNMT3A Polyclonal Antibody (A3169)

Immunofluorescence analysis of A549 cells using DNMT3A antibody (A3169).

* For research use only. Not for therapeutic or diagnostic purposes.

Inquire About This Product

Submit your question about A3169 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A3169? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
5 μL sample size available Add to Cart
Contact us to order