Product Type > Antibodies > Primary Antibodies > Methyl-specific Antibodies

Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155)

Review (1) Publications (4) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155)

Western blot analysis of various lysates using Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Dot Blot - Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155)

Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H3-R2 antibody (A3155).

ABclonal:Immunofluorescence - Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155)

Immunofluorescence analysis of HeLa cells using Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155)

Immunofluorescence analysis of MCF7 cells using Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155)

Immunofluorescence analysis of NIH/3T3 cells using Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155)

Immunofluorescence analysis of PC-12 cells using Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb(A3155)

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Overview

Product name Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb
Catalog No. A3155
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen A synthetic asymmetric dimethylated peptide around R2 of human histone H3 (NP_003520.1).
Sequence MARTK
Gene ID 82908350
Swiss prot Q16695P68431
Synonyms H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; Asymmetric DiMethyl-Histone H3-R2
Calculated MW 16kDa
Observed MW 17kDa

Applications

Reactivity Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples NIH/3T3, C6
Cellular location Chromosome, Nucleus
Customer validation

WB (HippocampusMus musculus, Homo sapiens, Mus musculus)

ICC (Homo sapiens)

IF (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb images

ABclonal:Western blot - Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155)}

Western blot - Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155)

Western blot analysis of various lysates using Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Dot Blot - Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155)}

Dot Blot - Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155)

Dot-blot analysis of all sorts of methylation peptides using Asymmetric DiMethyl-Histone H3-R2 antibody (A3155).
ABclonal:Immunofluorescence - Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155)}

Immunofluorescence - Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155)

Immunofluorescence analysis of HeLa cells using Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155)}

Immunofluorescence - Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155)

Immunofluorescence analysis of MCF7 cells using Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155)}

Immunofluorescence - Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155)

Immunofluorescence analysis of NIH/3T3 cells using Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155)}

Immunofluorescence - Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155)

Immunofluorescence analysis of PC-12 cells using Asymmetric DiMethyl-Histone H3-R2 Rabbit pAb (A3155) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on H3-4. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to H3-4. (Distance between topics and target gene indicate popularity.) H3-4

* Data provided by citexs.com, for reference only.

Publishing research using A3155? Please let us know so that we can cite the reference in this datasheet.

Antibodies (114)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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