Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

[One Step] eIF5A Antibody Kit (RK05645)

Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:

ABclonal:Western blot - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)

Western blot analysis of lysates from HeLa cells,using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)

Immunohistochemistry analysis of HRP Goat Anti-Rabbit IgG (H+L) in paraffin-embedded human testis tissue using HRP Goat Anti-Rabbit IgG (H+L) (AS014) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Western blot - eIF5A Rabbit pAb (A2016)

Western blot analysis of extracts of various cell lines, using eIF5A antibody (A2016) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunohistochemistry - eIF5A Rabbit pAb (A2016)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using eIF5A Rabbit pAb (A2016) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - eIF5A Rabbit pAb (A2016)

Immunofluorescence analysis of U2OS cells using eIF5A antibody (A2016). Blue: DAPI for nuclear staining.

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Overview

Product name [One Step] eIF5A Antibody Kit
Catalog No. RK05645
Host species
Purification method
Isotype

Background

Immunogen information

Immunogen
Sequence Email for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW

Applications

Reactivity
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
Storage buffer
Application key
Positive samples
Cellular location

[One Step] eIF5A Antibody Kit images

ABclonal:Western blot - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)}

Western blot - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)

Western blot analysis of lysates from HeLa cells,using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)}

Immunohistochemistry - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)

Immunohistochemistry analysis of HRP Goat Anti-Rabbit IgG (H+L) in paraffin-embedded human testis tissue using HRP Goat Anti-Rabbit IgG (H+L) (AS014) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Western blot - eIF5A Rabbit pAb (A2016)}

Western blot - eIF5A Rabbit pAb (A2016)

Western blot analysis of extracts of various cell lines, using eIF5A antibody (A2016) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - eIF5A Rabbit pAb (A2016)}

Immunohistochemistry - eIF5A Rabbit pAb (A2016)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using eIF5A Rabbit pAb (A2016) at dilution of 1:20 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - eIF5A Rabbit pAb (A2016)}

Immunofluorescence - eIF5A Rabbit pAb (A2016)

Immunofluorescence analysis of U2OS cells using eIF5A antibody (A2016). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on EIF5A. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to EIF5A. (Distance between topics and target gene indicate popularity.) EIF5A

* Data provided by citexs.com, for reference only.

Publishing research using RK05645? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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