Western blot - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)
Western blot analysis of lysates from HeLa cells,using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
Immunohistochemistry - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)
Immunohistochemistry analysis of HRP Goat Anti-Rabbit IgG (H+L) in paraffin-embedded human testis tissue using HRP Goat Anti-Rabbit IgG (H+L) (AS014) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
Western blot - [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248)
Western blot analysis of various lysates using [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Western blot - [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248)
Western blot analysis of lysates from wild type (WT) and HP1 gamma/HP1 gamma/CBX3 knockout (KO) 293T cells, using [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
Immunofluorescence - [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248)
Immunofluorescence analysis of C6 cells using [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence - [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248)
Immunofluorescence analysis of U-2 OS cells using [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunoprecipitation - [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248)
Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg HP1 gamma/CBX3 antibody (A2248). Western blot was performed from the immunoprecipitate using HP1 gamma/CBX3 antibody (A2248) at a dilution of 1:1000.