Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

[One Step] CBX3 Antibody Kit (RK05631)

Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:

ABclonal:Western blot - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)

Western blot analysis of lysates from HeLa cells,using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)

Immunohistochemistry analysis of HRP Goat Anti-Rabbit IgG (H+L) in paraffin-embedded human testis tissue using HRP Goat Anti-Rabbit IgG (H+L) (AS014) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Western blot - [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248)

Western blot analysis of various lysates using [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Western blot - [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248)

Western blot analysis of lysates from wild type (WT) and HP1 gamma/HP1 gamma/CBX3 knockout (KO) 293T cells, using [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248)

Immunofluorescence analysis of C6 cells using [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248)

Immunofluorescence analysis of U-2 OS cells using [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248)

Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg HP1 gamma/CBX3 antibody (A2248). Western blot was performed from the immunoprecipitate using HP1 gamma/CBX3 antibody (A2248) at a dilution of 1:1000.

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Overview

Product name [One Step] CBX3 Antibody Kit
Catalog No. RK05631
Host species
Purification method
Isotype

Background

Immunogen information

Immunogen
Sequence Email for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW

Applications

Reactivity
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
Storage buffer
Application key
Positive samples
Cellular location

[One Step] CBX3 Antibody Kit images

ABclonal:Western blot - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)}

Western blot - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)

Western blot analysis of lysates from HeLa cells,using GAPDH (AC001) antibody as the primary antibody at dilution of 1:80000.
Secondary antibody: using HRP Goat Anti-Rabbit IgG (H+L) antibody (AS014) at 1:4000-1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)}

Immunohistochemistry - HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014)

Immunohistochemistry analysis of HRP Goat Anti-Rabbit IgG (H+L) in paraffin-embedded human testis tissue using HRP Goat Anti-Rabbit IgG (H+L) (AS014) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Western blot - [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248)}

Western blot - [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248)

Western blot analysis of various lysates using [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Western blot - [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248)}

Western blot - [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248)

Western blot analysis of lysates from wild type (WT) and HP1 gamma/HP1 gamma/CBX3 knockout (KO) 293T cells, using [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248)}

Immunofluorescence - [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248)

Immunofluorescence analysis of C6 cells using [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248)}

Immunofluorescence - [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248)

Immunofluorescence analysis of U-2 OS cells using [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248)}

Immunoprecipitation - [KO Validated] HP1 gamma/CBX3 Rabbit pAb (A2248)

Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg HP1 gamma/CBX3 antibody (A2248). Western blot was performed from the immunoprecipitate using HP1 gamma/CBX3 antibody (A2248) at a dilution of 1:1000.

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Related Targets

Based on existing publications, the following genes are closely related to research on CBX3. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to CBX3. (Distance between topics and target gene indicate popularity.) CBX3

* Data provided by citexs.com, for reference only.

Publishing research using RK05631? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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