Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

Cardiac Troponin I (TNNI3) Rabbit mAb (A21246)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Cardiac Troponin I (TNNI3) Rabbit mAb (A21246)

Western blot analysis of lysates from Mouse heart, using Cardiac Troponin I (TNNI3) Rabbit mAb (A21246) at1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - Cardiac Troponin I (TNNI3) Rabbit mAb (A21246)

Immunofluorescence analysis of paraffin-embedded Rat heart using Cardiac Troponin I (TNNI3) Rabbit mAb (A21246) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Cardiac Troponin I (TNNI3) Rabbit mAb (A21246)

Immunofluorescence analysis of paraffin-embedded Mouse heart using Cardiac Troponin I (TNNI3) Rabbit mAb (A21246) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name Cardiac Troponin I (TNNI3) Rabbit mAb
Catalog No. A21246
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC5109-17

Background

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). Troponin I is useful in making a diagnosis of heart failure, and of ischemic heart disease. An elevated level of troponin is also now used as indicator of acute myocardial injury in patients hospitalized with moderate/severe Coronavirus Disease 2019 (COVID-19). Such elevation has also been associated with higher risk of mortality in cardiovascular disease patients hospitalized due to COVID-19.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-210 of human Cardiac Troponin I (TNNI3) (NP_000354.4).
Sequence MADGSSDAAREPRPAPAPIRRRSSNYRAYATEPHAKKKSKISASRKLQLKTLLLQIAKQELEREAEERRGEKGRALSTRCQPLELAGLGFAELQDLCRQLHARVDKVDEERYDIEAKVTKNITEIADLTQKIFDLRGKFKRPTLRRVRISADAMMQALLGARAKESLDLRAHLKQVKKEDTEKENREVGDWRKNIDALSGMEGRKKKFES
Gene ID 7137
Swiss prot P19429
Synonyms CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF; Cardiac Troponin I (TNNI3)
Calculated MW 24kDa
Observed MW 28kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications Testing results
IF/ICC MouseRat
WB MouseRat
Recommended dilution
  • WB 1:2000 - 1:10000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples Mouse heart
Cellular location cardiac myofibril, cytosol, sarcomere

Documents

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Research Area

Cardiac Troponin I (TNNI3) Rabbit mAb images

ABclonal:Western blot - Cardiac Troponin I (TNNI3) Rabbit mAb (A21246)}

Western blot - Cardiac Troponin I (TNNI3) Rabbit mAb (A21246)

Western blot analysis of lysates from Mouse heart, using Cardiac Troponin I (TNNI3) Rabbit mAb (A21246) at1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - Cardiac Troponin I (TNNI3) Rabbit mAb (A21246)}

Immunofluorescence - Cardiac Troponin I (TNNI3) Rabbit mAb (A21246)

Immunofluorescence analysis of paraffin-embedded Rat heart using Cardiac Troponin I (TNNI3) Rabbit mAb (A21246) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Cardiac Troponin I (TNNI3) Rabbit mAb (A21246)}

Immunofluorescence - Cardiac Troponin I (TNNI3) Rabbit mAb (A21246)

Immunofluorescence analysis of paraffin-embedded Mouse heart using Cardiac Troponin I (TNNI3) Rabbit mAb (A21246) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on TNNI3. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TNNI3. (Distance between topics and target gene indicate popularity.) TNNI3

* Data provided by citexs.com, for reference only.

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Antibodies (4)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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