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c-Myc Rabbit pAb (A1309)

Reviews (2)Publications (13) Datasheet

ABclonal:Western blot - c-Myc Polyclonal Antibody (A1309)

Western blot analysis of extracts of rat brain, using c-Myc antibody (A1309) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - c-Myc Polyclonal Antibody (A1309)

Western blot analysis of extracts of various cell lines, using c-Myc antibody (A1309) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Review
ABclonal: review for c-Myc Rabbit pAb(A1309)
Review
ABclonal: review for c-Myc Rabbit pAb(A1309)

Overview

Product namec-Myc Rabbit pAb
Catalog No.A1309
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human c-Myc (NP_002458.2).
SequenceMDFFRVVENQQPPATMPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGD
Gene ID4609
Swiss protP01106
SynonymsMRTL; MYCC; bHLHe39; c-Myc; MYC
Calculated MW48kDa/50kDa
Observed MW49kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa, HL-60, MCF7, Mouse liver
Cellular locationNucleus, nucleolus, nucleoplasm
Customer validation

(unknow, Human Bladder Cancer)

WB(Homo sapiens, Gallus gallus, Mus musculus, Sus scrofa)

Co-IP(Homo sapiens,Gallus gallus, Homo sapiens, Mus musculus)

ChIP(Homo sapiens,Mus musculus)

ABclonal:Western blot - c-Myc Polyclonal Antibody (A1309) }

Western blot - c-Myc Polyclonal Antibody (A1309)

Western blot analysis of extracts of rat brain, using c-Myc antibody (A1309) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - c-Myc Polyclonal Antibody (A1309) }

Western blot - c-Myc Polyclonal Antibody (A1309)

Western blot analysis of extracts of various cell lines, using c-Myc antibody (A1309) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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