Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A11111)

Publications (2) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A11111)

Western blot analysis of extracts of various cell lines, using α-Smooth Muscle Actin (ACTA2) antibody (A11111).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name α-Smooth Muscle Actin (ACTA2) Rabbit mAb
Catalog No. A11111
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome.

Immunogen information

Immunogen Recombinant protein of human α-Smooth Muscle Actin (ACTA2)
Sequence Email for sequence
Gene ID 59
Swiss prot P62736
Synonyms ACTSA; α-Smooth Muscle Actin (ACTA2)
Calculated MW 42kDa
Observed MW 42kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • FC 1:20 - 1:50
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Flow Cytometry    
Positive samples A-549, HeLa, NIH/3T3, Mouse heart
Cellular location Cytoplasm, cytoskeleton
Customer validation

WB (Mus musculus)

IF (Homo sapiens)

Research Area

α-Smooth Muscle Actin (ACTA2) Rabbit mAb images

ABclonal:Western blot - α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A11111)}

Western blot - α-Smooth Muscle Actin (ACTA2) Rabbit mAb (A11111)

Western blot analysis of extracts of various cell lines, using α-Smooth Muscle Actin (ACTA2) antibody (A11111).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on ACTA2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ACTA2. (Distance between topics and target gene indicate popularity.) ACTA2

* Data provided by citexs.com, for reference only.

Publishing research using A11111? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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