Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

ZNF281 Rabbit pAb (A12650)

Publications (2) Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - ZNF281 Rabbit pAb (A12650)

Western blot analysis of extracts of mouse spleen, using ZNF281 antibody (A12650) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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Overview

Product name ZNF281 Rabbit pAb
Catalog No. A12650
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Enables DNA-binding transcription repressor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Involved in negative regulation of gene expression; negative regulation of transcription by RNA polymerase II; and positive regulation of transcription, DNA-templated. Located in nucleoplasm.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 676-895 of human ZNF281 (NP_036614.1).
Sequence FEKSTNASFTLGHGFQFVSLSSPLHNHTLFPEKQIYTTSPLECGFGQSVTSVLPSSLPKPPFGMLFGSQPGLYLSALDATHQQLTPSQELDDLIDSQKNLETSSAFQSSSQKLTSQKEQKNLESSTGFQIPSQELASQIDPQKDIEPRTTYQIENFAQAFGSQFKSGSRVPMTFITNSNGEVDHRVRTSVSDFSGYTNMMSDVSEPCSTRVKTPTSQSYR
Gene ID 23528
Swiss prot Q9Y2X9
Synonyms GZP1; ZBP99; ZBP-99; ZNP-99; ZNF281
Calculated MW 97kDa
Observed MW 110kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:1000 - 1:3000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Mouse spleen
Cellular location Nucleus
Customer validation

WB (Mus musculus, Homo sapiens)

IF (Homo sapiens)

Research Area

ZNF281 Rabbit pAb images

ABclonal:Western blot - ZNF281 Rabbit pAb (A12650)}

Western blot - ZNF281 Rabbit pAb (A12650)

Western blot analysis of extracts of mouse spleen, using ZNF281 antibody (A12650) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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Related Targets

Based on existing publications, the following genes are closely related to research on ZNF281. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ZNF281. (Distance between topics and target gene indicate popularity.) ZNF281

* Data provided by citexs.com, for reference only.

Publishing research using A12650? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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