Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

ZNF20 Rabbit pAb (A7346)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - ZNF20 Rabbit pAb (A7346)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with ZNF20 using ZNF20 Rabbit pAb (A7346) at 1:700 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - ZNF20 Rabbit pAb (A7346)

Immunohistochemistry analysis of ZNF20 in paraffin-embedded human brain cancer using ZNF20 Rabbit pAb (A7346) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ZNF20 Rabbit pAb (A7346)

Immunohistochemistry analysis of ZNF20 in paraffin-embedded human kidney cancer using ZNF20 Rabbit pAb (A7346) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Overview

Product name ZNF20 Rabbit pAb
Catalog No. A7346
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Predicted to enable DNA-binding transcription repressor activity, RNA polymerase II-specific and RNA polymerase II transcription regulatory region sequence-specific DNA binding activity. Predicted to be involved in negative regulation of transcription by RNA polymerase II. Predicted to be located in nucleus.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ZNF20 (NP_066966.2).
Sequence MMFQDSVAFEDVAVSFTQEEWALLDPSQKNLYRDVMQETFKNLTSVGKTWKVQNIEDEYKNPRRNLSLMREKLCESKESHHCGESFNQIADDMLNRKTLPGITPCESSVCGEVGTGHSSLNTHIRADTGHKSSEYQEYGENPYRNKECKKAFSYLDSFQSHDKACTKEKPYDGKECTETFISHSCIQRHRVMHSGDGPYK
Gene ID 7568
Swiss prot P17024
Synonyms KOX13; ZNF20
Calculated MW 62kDa
Observed MW 62kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples 293T transfected with ZNF20
Cellular location Nucleus

Documents

Certificate of Compliance

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Lot number

Research Area

ZNF20 Rabbit pAb images

ABclonal:Western blot - ZNF20 Rabbit pAb (A7346)}

Western blot - ZNF20 Rabbit pAb (A7346)

Western blot analysis of lysates from wild type (WT) and 293T cells transfected with ZNF20 using ZNF20 Rabbit pAb (A7346) at 1:700 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - ZNF20 Rabbit pAb (A7346)}

Immunohistochemistry - ZNF20 Rabbit pAb (A7346)

Immunohistochemistry analysis of ZNF20 in paraffin-embedded human brain cancer using ZNF20 Rabbit pAb (A7346) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ZNF20 Rabbit pAb (A7346)}

Immunohistochemistry - ZNF20 Rabbit pAb (A7346)

Immunohistochemistry analysis of ZNF20 in paraffin-embedded human kidney cancer using ZNF20 Rabbit pAb (A7346) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on ZNF20. (Numbers indicate number of published articles where both targets appear.)

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Related Research Area

Based on existing publications, the following research topics are related to ZNF20. (Distance between topics and target gene indicate popularity.) ZNF20

* Data provided by citexs.com, for reference only.

Publishing research using A7346? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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