Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

ZMPSTE24 Rabbit pAb (A8858)

Publication (1) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - ZMPSTE24 Rabbit pAb (A8858)

Western blot analysis of extracts of A-431 cells, using ZMPSTE24 antibody (A8858).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name ZMPSTE24 Rabbit pAb
Catalog No. A8858
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the peptidase M48A family. The encoded protein is a zinc metalloproteinase involved in the two step post-translational proteolytic cleavage of carboxy terminal residues of farnesylated prelamin A to form mature lamin A. Mutations in this gene have been associated with mandibuloacral dysplasia and restrictive dermopathy.

Immunogen information

Immunogen A synthetic Peptide of human ZMPSTE24
Sequence Email for sequence
Gene ID 10269
Swiss prot O75844
Synonyms HGPS; PRO1; FACE1; RSDM1; STE24; FACE-1; Ste24p; ZMPSTE24
Calculated MW 55kDa
Observed MW 50kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    
Positive samples A-431
Cellular location Endoplasmic reticulum membrane, Multi-pass membrane protein, Nucleus inner membrane
Customer validation

IHC (Mus musculus)

Research Area

ZMPSTE24 Rabbit pAb images

ABclonal:Western blot - ZMPSTE24 Rabbit pAb (A8858)}

Western blot - ZMPSTE24 Rabbit pAb (A8858)

Western blot analysis of extracts of A-431 cells, using ZMPSTE24 antibody (A8858).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on ZMPSTE24. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ZMPSTE24. (Distance between topics and target gene indicate popularity.) ZMPSTE24

* Data provided by citexs.com, for reference only.

Publishing research using A8858? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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