Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

ZFYVE27 Rabbit pAb (A15943)

Publication (1) Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Rat

ABclonal:Western blot - ZFYVE27 Rabbit pAb (A15943)

Western blot analysis of lysates from DU145 cells, using ZFYVE27 Rabbit pAb (A15943) at 1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

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Overview

Product name ZFYVE27 Rabbit pAb
Catalog No. A15943
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a protein with several transmembrane domains, a Rab11-binding domain and a lipid-binding FYVE finger domain. The encoded protein appears to promote neurite formation. A mutation in this gene has been reported to be associated with hereditary spastic paraplegia, however the pathogenicity of the mutation, which may simply represent a polymorphism, is unclear.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 217-411 of human ZFYVE27 (NP_653189.3).
Sequence VEFFRVVSEYRASLQQRMNPKQEEHAFESPPPPDVGGKDGLMDSTPALTPTEDLTPGSVEEAEEAEPDEEFKDAIEETHLVVLEDDEGAPCPAEDELALQDNGFLSKNEVLRSKVSRLTERLRKRYPTNNFGNCTGCSATFSVLKKRRSCSNCGNSFCSRCCSFKVPKSSMGATAPEAQRETVFVCASCNQTLSK
Gene ID 118813
Swiss prot Q5T4F4
Synonyms SPG33; PROTRUDIN; ZFYVE27
Calculated MW 46kDa
Observed MW 46kDa

Applications

Reactivity Human, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples DU145
Cellular location Cell membrane, Cell projection, Endoplasmic reticulum membrane, Multi-pass membrane protein, Recycling endosome membrane, growth cone membrane
Customer validation

WB (Homo sapiens)

ZFYVE27 Rabbit pAb images

ABclonal:Western blot - ZFYVE27 Rabbit pAb (A15943)}

Western blot - ZFYVE27 Rabbit pAb (A15943)

Western blot analysis of lysates from DU145 cells, using ZFYVE27 Rabbit pAb (A15943) at 1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

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Related Targets

Based on existing publications, the following genes are closely related to research on ZFYVE27. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to ZFYVE27. (Distance between topics and target gene indicate popularity.) ZFYVE27

* Data provided by citexs.com, for reference only.

Publishing research using A15943? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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