Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

YWHAZ Rabbit pAb (A0174)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - YWHAZ Rabbit pAb (A0174)

Western blot analysis of extracts of various cell lines, using YWHAZ antibody (A0174).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name YWHAZ Rabbit pAb
Catalog No. A0174
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene.

Immunogen information

Immunogen A synthetic peptide of human YWHAZ
Sequence Email for sequence
Gene ID 7534
Swiss prot P63104
Synonyms HEL4; YWHAD; KCIP-1; HEL-S-3; POPCHAS; HEL-S-93; 14-3-3-zeta; YWHAZ
Calculated MW 28kDa
Observed MW 30kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • FC 1:20 - 1:50
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    Flow Cytometry    
Positive samples Mouse brain, SH-SY5Y
Cellular location Cytoplasm, Melanosome

Research Area

YWHAZ Rabbit pAb images

ABclonal:Western blot - YWHAZ Rabbit pAb (A0174)}

Western blot - YWHAZ Rabbit pAb (A0174)

Western blot analysis of extracts of various cell lines, using YWHAZ antibody (A0174).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on YWHAZ. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to YWHAZ. (Distance between topics and target gene indicate popularity.) YWHAZ

* Data provided by citexs.com, for reference only.

Publishing research using A0174? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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