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YAP1 Rabbit pAb (A1001)

Publications (2) Datasheet

ABclonal:Western blot - YAP1 Polyclonal Antibody (A1001)
ABclonal:Immunohistochemistry - YAP1 Polyclonal Antibody (A1001)
ABclonal:Immunohistochemistry - YAP1 Polyclonal Antibody (A1001)
ABclonal:Immunohistochemistry - YAP1 Polyclonal Antibody (A1001)
ABclonal:Immunofluorescence - YAP1 Polyclonal Antibody (A1001)
ABclonal:Immunofluorescence - YAP1 Polyclonal Antibody (A1001)

Overview

Product nameYAP1 Rabbit pAb
Catalog No.A1001
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 155-504 of human YAP1 (NP_001123617.1).
SequencePTAQHLRQSSFEIPDDVPLPAGWEMAKTSSGQRYFLNHIDQTTTWQDPRKAMLSQMNVTAPTSPPVQQNMMNSASGPLPDGWEQAMTQDGEIYYINHKNKTTSWLDPRLDPRFAMNQRISQSAPVKQPPPLAPQSPQGGVMGGSNSNQQQQMRLQQLQMEKERLRLKQQELLRQAMRNINPSTANSPKCQELALRSQLPTLEQDGGTQNPVSSPGMSQELRTMTTNSSDPFLNSGTYHSRDESTDSGLSMSSYSVPRTPDDFLNSVDEMDTGDTINQSTLPSQQNRFPDYLEAIPGTNVDLGTLEGDGMNIEGEELMPSLQEALSSDILNDMESVLAATKLDKESFLTWL
Gene ID10413
Swiss protP46937
SynonymsCOB1; YAP; YAP2; YAP65; YKI; YAP1
Calculated MW36kDa/48kDa/49kDa/50kDa/52kDa/53kDa/54kDa
Observed MW72kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesNIH/3T3, 293T, Rat liver, DU145, HeLa, BT-474, A-549, Mouse lung, Rat kidney
Cellular locationCytoplasm, Nucleus
Customer validation

WB(Mus musculus, Homo sapiens)

ABclonal:Western blot - YAP1 Polyclonal Antibody (A1001) }

Western blot - YAP1 Polyclonal Antibody (A1001)

Western blot analysis of extracts of various cell lines, using YAP1 antibody (A1001) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - YAP1 Polyclonal Antibody (A1001) }

Immunohistochemistry - YAP1 Polyclonal Antibody (A1001)

Immunohistochemistry of paraffin-embedded human liver cancer using YAP1 antibody (A1001) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - YAP1 Polyclonal Antibody (A1001) }

Immunohistochemistry - YAP1 Polyclonal Antibody (A1001)

Immunohistochemistry of paraffin-embedded human placenta using YAP1 antibody (A1001) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - YAP1 Polyclonal Antibody (A1001) }

Immunohistochemistry - YAP1 Polyclonal Antibody (A1001)

Immunohistochemistry of paraffin-embedded rat brain using YAP1 antibody (A1001) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - YAP1 Polyclonal Antibody (A1001) }

Immunofluorescence - YAP1 Polyclonal Antibody (A1001)

Immunofluorescence analysis of C6 cells using YAP1 antibody (A1001) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - YAP1 Polyclonal Antibody (A1001) }

Immunofluorescence - YAP1 Polyclonal Antibody (A1001)

Immunofluorescence analysis of NIH/3T3 cells using YAP1 antibody (A1001) at dilution of 1:100. Blue: DAPI for nuclear staining.

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