Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

Vimentin Rabbit mAb (A11883)

Publication (1) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Monkey

ABclonal:Western blot - Vimentin Rabbit mAb (A11883)

Western blot analysis of extracts of various cell lines, using Vimentin antibody (A11883).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name Vimentin Rabbit mAb
Catalog No. A11883
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.

Immunogen information

Immunogen Recombinant protein of human Vimentin
Sequence Email for sequence
Gene ID 7431
Swiss prot P08670
Synonyms CTRCT30; HEL113; Vimentin; VIM; vimentin
Calculated MW 54kDa
Observed MW 54kDa

Applications

Reactivity Human, Mouse, Rat, Monkey
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:50 - 1:100
  • FC 1:20 - 1:50
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Flow Cytometry    
Positive samples HeLa, NIH/3T3, C6, COS-7
Cellular location Cytoplasm
Customer validation

WB (Mus musculus)

Research Area

Vimentin Rabbit mAb images

ABclonal:Western blot - Vimentin Rabbit mAb (A11883)}

Western blot - Vimentin Rabbit mAb (A11883)

Western blot analysis of extracts of various cell lines, using Vimentin antibody (A11883).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on VIM. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to VIM. (Distance between topics and target gene indicate popularity.) VIM

* Data provided by citexs.com, for reference only.

Publishing research using A11883? Please let us know so that we can cite the reference in this datasheet.

Antibodies (8)

ELISA Kits (1)

Proteins (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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