Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

VRK2 Rabbit pAb (A12243)

Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - VRK2 Rabbit pAb (A12243)

Western blot analysis of extracts of LO2 cells, using VRK2 antibody (A12243) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Overview

Product name VRK2 Rabbit pAb
Catalog No. A12243
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. The encoded protein acts as an effector of signaling pathways that regulate apoptosis and tumor cell growth. Variants in this gene have been associated with schizophrenia. Alternative splicing results in multiple transcript variants that differ in their subcellular localization and biological activity.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 269-508 of human VRK2 (NP_006287.2).
Sequence DELPQSVLKWAPSGSSCCEIAQFLVCAHSLAYDEKPNYQALKKILNPHGIPLGPLDFSTKGQSINVHTPNSQKVDSQKAATKQVNKAHNRLIEKKVHSERSAESCATWKVQKEEKLIGLMNNEAAQESTRRRQKYQESQEPLNEVNSFPQKISYTQFPNSFYEPHQDFTSPDIFKKSRSPSWYKYTSTVSTGITDLESSTGLWPTISQFTLSEETNADVYYYRIIIPVLLMLVFLALFFL
Gene ID 7444
Swiss prot Q86Y07
Synonyms VRK2
Calculated MW 58kDa
Observed MW 54kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples LO2
Cellular location Cytoplasm, Endoplasmic reticulum membrane, Mitochondrion membrane, Nucleus, Single-pass type IV membrane protein

VRK2 Rabbit pAb images

ABclonal:Western blot - VRK2 Rabbit pAb (A12243)}

Western blot - VRK2 Rabbit pAb (A12243)

Western blot analysis of extracts of LO2 cells, using VRK2 antibody (A12243) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on VRK2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to VRK2. (Distance between topics and target gene indicate popularity.) VRK2

* Data provided by citexs.com, for reference only.

Publishing research using A12243? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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