Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

VGLL4 Rabbit pAb (A10578)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - VGLL4 Rabbit pAb (A10578)

Western blot analysis of lysates from mouse thymus, using VGLL4 Rabbit pAb (A10578) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Overview

Product name VGLL4 Rabbit pAb
Catalog No. A10578
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Predicted to enable transcription coactivator binding activity. Involved in several processes, including negative regulation of cardiac muscle cell proliferation; negative regulation of hippo signaling; and positive regulation of cellular protein catabolic process. Located in nucleus. Is expressed in lung; submandibular gland primordium; tooth; and vibrissa. Orthologous to human VGLL4 (vestigial like family member 4).

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-287 of mouse VGLL4 (NP_808351.1).
Sequence MLFMKMDLLNYQYLDKMNNNIGVLCYEGEASLRGEPRMQTLPVASALSSHRTGPPPISPSKRKFSMEPGDKDLDCENDHVSKMSRIFSPHLNKTVNGDCRRDPRERSRSPIERAAAPAVSLHGGHLYASLPSLMEQPLALTKNSSDTGRSAVERQQNRPSVITCASAGARNCNLSHCPIAHSGCSAPGSASYRRPPSATATCDPVVEEHFRRSLGKNYKEPEPAPNSVSITGSVDDHFAKALGDTWLQIKAAKDSASSSPESASRRGQPASPTAHMVSHSHSPSVVS
Gene ID 232334
Swiss prot Q80V24
Synonyms VGL-4; VGLL4
Calculated MW 31kDa
Observed MW 31kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Mouse thymus
Cellular location Nucleus

Documents

Certificate of Compliance

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VGLL4 Rabbit pAb images

ABclonal:Western blot - VGLL4 Rabbit pAb (A10578)}

Western blot - VGLL4 Rabbit pAb (A10578)

Western blot analysis of lysates from mouse thymus, using VGLL4 Rabbit pAb (A10578) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Related Targets

Based on existing publications, the following genes are closely related to research on Vgll4. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to Vgll4. (Distance between topics and target gene indicate popularity.) Vgll4

* Data provided by citexs.com, for reference only.

Publishing research using A10578? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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