Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

VDR Rabbit pAb (A11743)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - VDR Rabbit pAb (A11743)

Western blot analysis of extracts of various cell lines, using VDR antibody (A11743) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - VDR Rabbit pAb (A11743)

Immunofluorescence analysis of HeLa cells using VDR Rabbit pAb (A11743) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - VDR Rabbit pAb (A11743)

Immunofluorescence analysis of NIH/3T3 cells using VDR Rabbit pAb (A11743) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - VDR Rabbit pAb (A11743)

Immunofluorescence analysis of U2OS cells using VDR Rabbit pAb (A11743) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Overview

Product name VDR Rabbit pAb
Catalog No. A11743
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes vitamin D3 receptor, which is a member of the nuclear hormone receptor superfamily of ligand-inducible transcription factors. This receptor also functions as a receptor for the secondary bile acid, lithocholic acid. Downstream targets of vitamin D3 receptor are principally involved in mineral metabolism, though this receptor regulates a variety of other metabolic pathways, such as those involved in immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human VDR (NP_000367.1).
Sequence MEAMAASTSLPDPGDFDRNVPRICGVCGDRATGFHFNAMTCEGCKGFFRRSMKRKALFTCPFNGDCRITKDNRRHCQACRLKRCVDIGMMKEFILTDEEVQRKREMILKRKEEEALKDSLRPKLSEEQQRIIAILLDAHHKTYDPTYSDFCQFRPPVRVNDGGGSHPSRPNSRHTPSFSGDSSSSCSDHCITSSDMMDSSSFSNLDLSEEDSDDPSVTLELSQLSMLPHLADLVSYSIQKVIGFAKMIPGFRDLTSEDQIVLLKSSAIEVIMLRSNESFTMDDMSWTCGNQDYKYRVSDV
Gene ID 7421
Swiss prot P11473
Synonyms NR1I1; PPP1R163; VDR
Calculated MW 48kDa
Observed MW 48kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HeLa, HT-29
Cellular location Nucleus

Documents

Certificate of Compliance

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Lot number

Research Area

VDR Rabbit pAb images

ABclonal:Western blot - VDR Rabbit pAb (A11743)}

Western blot - VDR Rabbit pAb (A11743)

Western blot analysis of extracts of various cell lines, using VDR antibody (A11743) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - VDR Rabbit pAb (A11743)}

Immunofluorescence - VDR Rabbit pAb (A11743)

Immunofluorescence analysis of HeLa cells using VDR Rabbit pAb (A11743) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - VDR Rabbit pAb (A11743)}

Immunofluorescence - VDR Rabbit pAb (A11743)

Immunofluorescence analysis of NIH/3T3 cells using VDR Rabbit pAb (A11743) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - VDR Rabbit pAb (A11743)}

Immunofluorescence - VDR Rabbit pAb (A11743)

Immunofluorescence analysis of U2OS cells using VDR Rabbit pAb (A11743) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on VDR. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to VDR. (Distance between topics and target gene indicate popularity.) VDR

* Data provided by citexs.com, for reference only.

Publishing research using A11743? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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