Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

VDAC1 / Porin Rabbit pAb (A15735)

Publications (3) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - VDAC1 / Porin Rabbit pAb (A15735)

Western blot analysis of various lysates using VDAC1 / Porin Rabbit pAb (A15735) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - VDAC1 / Porin Rabbit pAb (A15735)

Immunohistochemistry analysis of VDAC1 / Porin in paraffin-embedded rat heart using VDAC1 / Porin Rabbit pAb (A15735) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - VDAC1 / Porin Rabbit pAb (A15735)

Immunohistochemistry analysis of VDAC1 / Porin in paraffin-embedded human colon carcinoma using VDAC1 / Porin Rabbit pAb (A15735) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - VDAC1 / Porin Rabbit pAb (A15735)

Immunohistochemistry analysis of VDAC1 / Porin in paraffin-embedded human stomach using VDAC1 / Porin Rabbit pAb (A15735) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Overview

Product name VDAC1 / Porin Rabbit pAb
Catalog No. A15735
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a voltage-dependent anion channel protein that is a major component of the outer mitochondrial membrane. The encoded protein facilitates the exchange of metabolites and ions across the outer mitochondrial membrane and may regulate mitochondrial functions. This protein also forms channels in the plasma membrane and may be involved in transmembrane electron transport. Alternate splicing results in multiple transcript variants. Multiple pseudogenes of this gene are found on chromosomes 1, 2 3, 6, 9, 12, X and Y.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human VDAC1 / Porin (NP_003365.1).
Sequence MAVPPTYADLGKSARDVFTKGYGFGLIKLDLKTKSENGLEFTSSGSANTETTKVTGSLETKYRWTEYGLTFTEKWNTDNTLGTEITVEDQLARGLKLTFDSSFSPNTGKKNAKIKTGYKREHINLGCDMDFDIAGPSIRGALVLGYEGWLAGYQMNFETAKSRVTQSNFAVGYKTDEFQLHTNVNDGTEFGGSIYQKVNKKLETAVNLAWTAGNSNTRFGIAAKYQIDPDACFSAKVNNSSLIGLGYTQTLKPGIKLTLSALLDGKNVNAGGHKLGLGLEFQA
Gene ID 7416
Swiss prot P21796
Synonyms PORIN; VDAC-1; VDAC1 / Porin
Calculated MW 31kDa
Observed MW 36kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples LO2, A-549, A-431, Raji, Rat heart, Rat brain
Cellular location Cell membrane, Membrane raft, Mitochondrion outer membrane, Multi-pass membrane protein
Customer validation

WB (Rattus norvegicus, Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

VDAC1 / Porin Rabbit pAb images

ABclonal:Western blot - VDAC1 / Porin Rabbit pAb (A15735)}

Western blot - VDAC1 / Porin Rabbit pAb (A15735)

Western blot analysis of various lysates using VDAC1 / Porin Rabbit pAb (A15735) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - VDAC1 / Porin Rabbit pAb (A15735)}

Immunohistochemistry - VDAC1 / Porin Rabbit pAb (A15735)

Immunohistochemistry analysis of VDAC1 / Porin in paraffin-embedded rat heart using VDAC1 / Porin Rabbit pAb (A15735) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - VDAC1 / Porin Rabbit pAb (A15735)}

Immunohistochemistry - VDAC1 / Porin Rabbit pAb (A15735)

Immunohistochemistry analysis of VDAC1 / Porin in paraffin-embedded human colon carcinoma using VDAC1 / Porin Rabbit pAb (A15735) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - VDAC1 / Porin Rabbit pAb (A15735)}

Immunohistochemistry - VDAC1 / Porin Rabbit pAb (A15735)

Immunohistochemistry analysis of VDAC1 / Porin in paraffin-embedded human stomach using VDAC1 / Porin Rabbit pAb (A15735) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on VDAC1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to VDAC1. (Distance between topics and target gene indicate popularity.) VDAC1

* Data provided by citexs.com, for reference only.

Publishing research using A15735? Please let us know so that we can cite the reference in this datasheet.

Antibodies (4)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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