Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

VAMP8 Rabbit pAb (A13915)

Publications (2) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - VAMP8 Rabbit pAb (A13915)

Western blot analysis of various lysates using VAMP8 Rabbit pAb (A13915) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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Overview

Product name VAMP8 Rabbit pAb
Catalog No. A13915
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes an integral membrane protein that belongs to the synaptobrevin/vesicle-associated membrane protein subfamily of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). The encoded protein is involved in the fusion of synaptic vesicles with the presynaptic membrane.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-75 of human VAMP8 (NP_003752.2).
Sequence MEEASEGGGNDRVRNLQSEVEGVKNIMTQNVERILARGENLEHLRNKTEDLEATSEHFKTTSQKVARKFWWKNVK
Gene ID 8673
Swiss prot Q9BV40
Synonyms EDB; VAMP-8; VAMP8
Calculated MW 11kDa
Observed MW 11kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HeLa, NIH/3T3, PC-12, Mouse kidney, Mouse liver, Rat lung
Cellular location Cell membrane, Early endosome membrane, Late endosome membrane, Lysosome membrane, Single-pass type IV membrane protein
Customer validation

WB (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

VAMP8 Rabbit pAb images

ABclonal:Western blot - VAMP8 Rabbit pAb (A13915)}

Western blot - VAMP8 Rabbit pAb (A13915)

Western blot analysis of various lysates using VAMP8 Rabbit pAb (A13915) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

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Related Targets

Based on existing publications, the following genes are closely related to research on VAMP8. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to VAMP8. (Distance between topics and target gene indicate popularity.) VAMP8

* Data provided by citexs.com, for reference only.

Publishing research using A13915? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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