Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

USP11 Mouse mAb (A4208)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - USP11 Mouse mAb (A4208)

Western blot analysis of extracts of human testis, using USP11 antibody (A4208).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name USP11 Mouse mAb
Catalog No. A4208
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

Protein ubiquitination controls many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. This dynamic process, involving ubiquitin conjugating enzymes and deubiquitinating enzymes, adds and removes ubiquitin. Deubiquitinating enzymes are cysteine proteases that specifically cleave ubiquitin from ubiquitin-conjugated protein substrates. This gene encodes a deubiquitinating enzyme which lies in a gene cluster on chromosome Xp11.23

Immunogen information

Immunogen A synthetic peptide of human USP11
Sequence Email for sequence
Gene ID 8237
Swiss prot P51784
Synonyms UHX1; USP11
Calculated MW 110kDa
Observed MW 110kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • FC 1:20 - 1:50
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    Flow Cytometry    
Positive samples human testis
Cellular location Cytoplasm, Nucleus

Research Area

USP11 Mouse mAb images

ABclonal:Western blot - USP11 Mouse mAb (A4208)}

Western blot - USP11 Mouse mAb (A4208)

Western blot analysis of extracts of human testis, using USP11 antibody (A4208).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on USP11. (Numbers indicate number of published articles where both targets appear.)

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Related Research Area

Based on existing publications, the following research topics are related to USP11. (Distance between topics and target gene indicate popularity.) USP11

* Data provided by citexs.com, for reference only.

Publishing research using A4208? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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