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UBE2I Polyclonal Antibody (A2193)

Datasheet

ABclonal:Western blot - UBE2I Polyclonal Antibody (A2193)
ABclonal:Immunohistochemistry - UBE2I Polyclonal Antibody (A2193)
ABclonal:Immunohistochemistry - UBE2I Polyclonal Antibody (A2193)

Overview

Product nameUBE2I Polyclonal Antibody
Catalog No.A2193
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Four alternatively spliced transcript variants encoding the same protein have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-158 of human UBE2I (NP_003336.1).
SequenceMSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS
Gene ID7329
Swiss protP63279
SynonymsC358B7.1; P18; UBC9
Calculated MW18kDa
Observed MW18kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samples293T, LO2, A-549, U-251MG, MCF-7, Mouse spleen, Mouse kidney, Rat spleen
Cellular locationCytoplasm, Nucleus
ABclonal:Western blot - UBE2I Polyclonal Antibody (A2193) }

Western blot - UBE2I Polyclonal Antibody (A2193)

Western blot analysis of extracts of various cell lines, using UBE2I antibody (A2193) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - UBE2I Polyclonal Antibody (A2193) }

Immunohistochemistry - UBE2I Polyclonal Antibody (A2193)

Immunohistochemistry of paraffin-embedded rat brain using UBE2I antibody (A2193) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - UBE2I Polyclonal Antibody (A2193) }

Immunohistochemistry - UBE2I Polyclonal Antibody (A2193)

Immunohistochemistry of paraffin-embedded mouse kidney using UBE2I antibody (A2193) at dilution of 1:100 (40x lens).

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