Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

UBA1 Rabbit pAb (A0673)

Review (1) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - UBA1 Rabbit pAb (A0673)

Western blot analysis of various lysates using UBA1 Rabbit pAb (A0673) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Review
ABclonal: review for UBA1 Rabbit pAb(A0673)

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Overview

Product name UBA1 Rabbit pAb
Catalog No. A0673
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11.23. Alternatively spliced transcript variants that encode the same protein have been described.

Immunogen information

Immunogen A synthetic peptide of human UBA1
Sequence Email for sequence
Gene ID 7317
Swiss prot P22314
Synonyms A1S9; A1ST; GXP1; UBE1; A1S9T; AMCX1; POC20; SMAX2; UBA1A; UBE1X; VEXAS; CFAP124; UBA1
Calculated MW 118kDa
Observed MW 118kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:100
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples HeLa, Jurkat, U-87MG, Mouse stomach, Mouse spleen, Mouse liver
Cellular location Cytoplasm, Mitochondrion, Nucleus, Nucleus
Customer validation

WB (A172)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

UBA1 Rabbit pAb images

ABclonal:Western blot - UBA1 Rabbit pAb (A0673)}

Western blot - UBA1 Rabbit pAb (A0673)

Western blot analysis of various lysates using UBA1 Rabbit pAb (A0673) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

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Related Targets

Based on existing publications, the following genes are closely related to research on UBA1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to UBA1. (Distance between topics and target gene indicate popularity.) UBA1

* Data provided by citexs.com, for reference only.

Publishing research using A0673? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Proteins (2)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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