Product Type > Antibodies > Primary Antibodies > Methyl-specific Antibodies

TriMethyl-Histone H4-K20 Rabbit pAb (A2372)

Review (1) Publications (5) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - TriMethyl-Histone H4-K20 Rabbit pAb (A2372)

Western blot analysis of various lysates, using TriMethyl-Histone H4-K20 Rabbit pAb (A2372) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Dot Blot - TriMethyl-Histone H4-K20 Rabbit pAb (A2372)

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H4-K20 antibody (A2372).

ABclonal:Immunohistochemistry - TriMethyl-Histone H4-K20 Rabbit pAb (A2372)

Immunohistochemistry analysis of TriMethyl-Histone H4-K20 in paraffin-embedded rat testis using TriMethyl-Histone H4-K20 Rabbit pAb (A2372) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - TriMethyl-Histone H4-K20 Rabbit pAb (A2372)

Immunohistochemistry analysis of TriMethyl-Histone H4-K20 in paraffin-embedded mouse testis using TriMethyl-Histone H4-K20 Rabbit pAb (A2372) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - TriMethyl-Histone H4-K20 Rabbit pAb (A2372)

Immunofluorescence analysis of 293T cells using TriMethyl-Histone H4-K20 Rabbit pAb (A2372). Blue: DAPI for nuclear staining.

Review
ABclonal: review for TriMethyl-Histone H4-K20 Rabbit pAb(A2372)

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Overview

Product name TriMethyl-Histone H4-K20 Rabbit pAb
Catalog No. A2372
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.

Immunogen information

Immunogen A synthetic trimethylated peptide around K20 of human histone H4 (NP_003529.1).
Sequence HRKVL
Gene ID 8359
Swiss prot P62805
Synonyms H4; H4/n; H4C1; H4C2; H4C3; H4C4; H4C5; H4C6; H4C8; H4C9; H4F2; H4FN; FO108; H4-16; H4C11; H4C12; H4C13; H4C15; H4C16; HIST2H4; HIST2H4A; TriMethyl-Histone H4-K20
Calculated MW 11kDa
Observed MW 11kDa

Applications

Reactivity Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • DB 1:500 - 1:2000
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa, NIH/3T3, C6, H4
Cellular location Chromosome, Nucleus
Customer validation

WB (Mouse uterusMus musculus, Eurosta solidaginis)

IF (Capra hircus, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

TriMethyl-Histone H4-K20 Rabbit pAb images

ABclonal:Western blot - TriMethyl-Histone H4-K20 Rabbit pAb (A2372)}

Western blot - TriMethyl-Histone H4-K20 Rabbit pAb (A2372)

Western blot analysis of various lysates, using TriMethyl-Histone H4-K20 Rabbit pAb (A2372) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Dot Blot - TriMethyl-Histone H4-K20 Rabbit pAb (A2372)}

Dot Blot - TriMethyl-Histone H4-K20 Rabbit pAb (A2372)

Dot-blot analysis of all sorts of methylation peptides using TriMethyl-Histone H4-K20 antibody (A2372).
ABclonal:Immunohistochemistry - TriMethyl-Histone H4-K20 Rabbit pAb (A2372)}

Immunohistochemistry - TriMethyl-Histone H4-K20 Rabbit pAb (A2372)

Immunohistochemistry analysis of TriMethyl-Histone H4-K20 in paraffin-embedded rat testis using TriMethyl-Histone H4-K20 Rabbit pAb (A2372) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - TriMethyl-Histone H4-K20 Rabbit pAb (A2372)}

Immunohistochemistry - TriMethyl-Histone H4-K20 Rabbit pAb (A2372)

Immunohistochemistry analysis of TriMethyl-Histone H4-K20 in paraffin-embedded mouse testis using TriMethyl-Histone H4-K20 Rabbit pAb (A2372) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - TriMethyl-Histone H4-K20 Rabbit pAb (A2372)}

Immunofluorescence - TriMethyl-Histone H4-K20 Rabbit pAb (A2372)

Immunofluorescence analysis of 293T cells using TriMethyl-Histone H4-K20 Rabbit pAb (A2372). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on H4C14. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to H4C14. (Distance between topics and target gene indicate popularity.) H4C14

* Data provided by citexs.com, for reference only.

Publishing research using A2372? Please let us know so that we can cite the reference in this datasheet.

Antibodies (33)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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