Product Type > Antibodies > Primary Antibodies > Methyl-specific Antibodies

TriMethyl-Histone H3-K64 Rabbit pAb (A7259)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat, Other (Wide Range Predicted)

ABclonal:Western blot - TriMethyl-Histone H3-K64 Rabbit pAb (A7259)

Western blot analysis of various lysates using TriMethyl-Histone H3-K64 Rabbit pAb (A7259) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 150s.

ABclonal:Western blot - TriMethyl-Histone H3-K64 Rabbit pAb (A7259)

Western blot analysis of various lysates using TriMethyl-Histone H3-K64 Rabbit pAb (A7259) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 120s.

ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K64 Rabbit pAb (A7259)

Immunohistochemistry analysis of TriMethyl-Histone H3-K64 in paraffin-embedded rat brain using TriMethyl-Histone H3-K64 Rabbit pAb (A7259) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K64 Rabbit pAb (A7259)

Immunohistochemistry analysis of TriMethyl-Histone H3-K64 in paraffin-embedded human breast using TriMethyl-Histone H3-K64 Rabbit pAb (A7259) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K64 Rabbit pAb (A7259)

Immunohistochemistry analysis of TriMethyl-Histone H3-K64 in paraffin-embedded mouse brain using TriMethyl-Histone H3-K64 Rabbit pAb (A7259) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - TriMethyl-Histone H3-K64 Rabbit pAb (A7259)

Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K64 Rabbit pAb (A7259) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - TriMethyl-Histone H3-K64 Rabbit pAb (A7259)

Immunofluorescence analysis of NIH/3T3 cells using TriMethyl-Histone H3-K64 Rabbit pAb (A7259) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name TriMethyl-Histone H3-K64 Rabbit pAb
Catalog No. A7259
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.

Immunogen information

Immunogen A synthetic trimethylated peptide around K56 of human TriMethyl-Histone H3-K64 (NP_003520.1).
Sequence YQKST
Gene ID 82908350
Swiss prot Q16695P68431
Synonyms H3t; H3.4; H3/g; H3FT; H3C16; HIST3H3; TriMethyl-Histone H3-K64
Calculated MW 16kDa
Observed MW 16kDa/17kDa

Applications

Reactivity Human, Mouse, Rat, Other (Wide Range Predicted)
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples HeLa, NIH/3T3, C6
Cellular location Chromosome, Nucleus

Documents

Certificate of Compliance

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TriMethyl-Histone H3-K64 Rabbit pAb images

ABclonal:Western blot - TriMethyl-Histone H3-K64 Rabbit pAb (A7259)}

Western blot - TriMethyl-Histone H3-K64 Rabbit pAb (A7259)

Western blot analysis of various lysates using TriMethyl-Histone H3-K64 Rabbit pAb (A7259) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 150s.
ABclonal:Western blot - TriMethyl-Histone H3-K64 Rabbit pAb (A7259)}

Western blot - TriMethyl-Histone H3-K64 Rabbit pAb (A7259)

Western blot analysis of various lysates using TriMethyl-Histone H3-K64 Rabbit pAb (A7259) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 120s.
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K64 Rabbit pAb (A7259)}

Immunohistochemistry - TriMethyl-Histone H3-K64 Rabbit pAb (A7259)

Immunohistochemistry analysis of TriMethyl-Histone H3-K64 in paraffin-embedded rat brain using TriMethyl-Histone H3-K64 Rabbit pAb (A7259) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K64 Rabbit pAb (A7259)}

Immunohistochemistry - TriMethyl-Histone H3-K64 Rabbit pAb (A7259)

Immunohistochemistry analysis of TriMethyl-Histone H3-K64 in paraffin-embedded human breast using TriMethyl-Histone H3-K64 Rabbit pAb (A7259) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - TriMethyl-Histone H3-K64 Rabbit pAb (A7259)}

Immunohistochemistry - TriMethyl-Histone H3-K64 Rabbit pAb (A7259)

Immunohistochemistry analysis of TriMethyl-Histone H3-K64 in paraffin-embedded mouse brain using TriMethyl-Histone H3-K64 Rabbit pAb (A7259) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K64 Rabbit pAb (A7259)}

Immunofluorescence - TriMethyl-Histone H3-K64 Rabbit pAb (A7259)

Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K64 Rabbit pAb (A7259) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - TriMethyl-Histone H3-K64 Rabbit pAb (A7259)}

Immunofluorescence - TriMethyl-Histone H3-K64 Rabbit pAb (A7259)

Immunofluorescence analysis of NIH/3T3 cells using TriMethyl-Histone H3-K64 Rabbit pAb (A7259) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on H3-4. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to H3-4. (Distance between topics and target gene indicate popularity.) H3-4

* Data provided by citexs.com, for reference only.

Publishing research using A7259? Please let us know so that we can cite the reference in this datasheet.

Antibodies (114)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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