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β-Tubulin Polyclonal Antibody (AC008)

Publications (9) Datasheet

ABclonal:Western blot - β-Tubulin Polyclonal Antibody (AC008)
ABclonal:Western blot - β-Tubulin Polyclonal Antibody (AC008)

Overview

Product nameβ-Tubulin Polyclonal Antibody
Catalog No.AC008
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a beta tubulin protein. This protein forms a dimer with alpha tubulin and acts as a structural component of microtubules. Mutations in this gene cause cortical dysplasia, complex, with other brain malformations 6. Alternative splicing results in multiple splice variants. There are multiple pseudogenes for this gene on chromosomes 1, 6, 7, 8, 9, and 13.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 40-260 of human β-Tubulin (NP_006077.2).
SequenceSDLQLERISVYYNEASSHKYVPRAILVDLEPGTMDSVRSGAFGHLFRPDNFIFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKECENCDCLQGFQLTHSLGGGTGSGMGTLLISKVREEYPDRIMNTFSVVPSPKVSDTVVEPYNATLSIHQLVENTDETYCIDNEALYDICFRTLKLATPTYGDLNHLVSATMSGVTTSLRFPGQLNADLRKLAVNMVPF
Gene ID203068
Swiss protP07437
SynonymsTUBB; CDCBM6; CSCSC1; M40; OK/SW-cl.56; TUBB1; TUBB5; tubulin beta chain
Calculated MW50kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:5000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesBT474, HeLa, U251, MCF7, mouse brain, mouse spinal cord, rat brain, rat spinal cord, HeLa
Cellular locationCytoplasm, cytoskeleton
Customer validation

WB(Crab, Oyster, Mouse, Human, Rat)

IHC(Rat)

IF(Mouse)

ABclonal:Western blot - β-Tubulin Polyclonal Antibody (AC008) }

Western blot - β-Tubulin Polyclonal Antibody (AC008)

Western blot analysis of extracts of various cell lines, using β-Tubulin antibody (AC008) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Western blot - β-Tubulin Polyclonal Antibody (AC008) }

Western blot - β-Tubulin Polyclonal Antibody (AC008)

Western blot analysis of HeLa cell using β-tubulin antibody (AC008) at different dilution. Each lane was loaded with 10 ug cell lysate.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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