Product Type > Antibodies > Secondary Antibodies

TRITC-conjugated Goat anti-Mouse IgG (H+L) (AS026)

Publications (8) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:

ABclonal:Immunofluorescence - TRITC-conjugated Goat anti-Mouse IgG (H+L) (AS026)

Immunofluorescence analysis of NIH/3T3 cells using TRITC Goat Anti-Mouse IgG (H+L) (AS026) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Flow CytoMetry - TRITC-conjugated Goat anti-Mouse IgG (H+L) (AS026)

Flow cytometric analysis of Positive antibody Human Calcitonin R (2.5μg/mL) in various cells (orange) compare to Mouse isotype control (blue) and non-staining control (Red). The secondary antibody used was TRITC Goat Anti-Mouse IgG (H+L) (AS026) at 1:100.

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Overview

Product name TRITC-conjugated Goat anti-Mouse IgG (H+L)
Catalog No. AS026
Host species Goat
Purification method Affinity purification
Isotype TRITC conjugated IgG

Background

Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.

Immunogen information

Immunogen Mouse IgG
Sequence Email for sequence
Gene ID
Swiss prot
Synonyms
Calculated MW
Observed MW

Applications

Reactivity
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • IF/ICC 1:50 - 1:200
  • FC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.025% Sodium Azide, 0.75% BSA, 50% glycerol, pH7.3.
Application key Immunofluorescence    Flow Cytometry    
Positive samples
Cellular location
Customer validation

IF (Oryza sativa, Homo sapiens, Rattus norvegicus, Mus musculus)

WB (Sus scrofa)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

TRITC-conjugated Goat anti-Mouse IgG (H+L) images

ABclonal:Immunofluorescence - TRITC-conjugated Goat anti-Mouse IgG (H+L) (AS026)}

Immunofluorescence - TRITC-conjugated Goat anti-Mouse IgG (H+L) (AS026)

Immunofluorescence analysis of NIH/3T3 cells using TRITC Goat Anti-Mouse IgG (H+L) (AS026) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Flow CytoMetry - TRITC-conjugated Goat anti-Mouse IgG (H+L) (AS026)}

Flow CytoMetry - TRITC-conjugated Goat anti-Mouse IgG (H+L) (AS026)

Flow cytometric analysis of Positive antibody Human Calcitonin R (2.5μg/mL) in various cells (orange) compare to Mouse isotype control (blue) and non-staining control (Red). The secondary antibody used was TRITC Goat Anti-Mouse IgG (H+L) (AS026) at 1:100.

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Related Targets

Based on existing publications, the following genes are closely related to research on Goat Anti-Mouse. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to Goat Anti-Mouse. (Distance between topics and target gene indicate popularity.) Goat Anti-Mouse

* Data provided by citexs.com, for reference only.

Publishing research using AS026? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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