Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

TRAPPC9 Rabbit pAb (A15527)

Datasheet

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Mouse

ABclonal:Western blot - TRAPPC9 Rabbit pAb (A15527)

Western blot analysis of lysates from mouse pancreas, using TRAPPC9 Rabbit pAb (A15527) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Overview

Product name TRAPPC9 Rabbit pAb
Catalog No. A15527
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a protein that likely plays a role in NF-kappa-B signaling. Mutations in this gene have been associated with autosomal-recessive cognitive disability. Alternatively spliced transcript variants have been described.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 947-1246 of human TRAPPC9 (NP_113654.4).
Sequence SHVKTLEAVLNFKYSGGPGHTEGYYRNLSLGLHVEVEPSVFFTRVSTLPATSTRQCHLLLDVFNSTEHELTVSTRSSEALILHAGECQRMAIQVDKFNFESFPESPGEKGQFANPKQLEEERREARGLEIHSKLGICWRIPSLKRSGEASVEGLLNQLVLEHLQLAPLQWDVLVDGQPCDREAVAACQVGDPVRLEVRLTNRSPRSVGPFALTVVPFQDHQNGVHNYDLHDTVSFVGSSTFYLDAVQPSGQSACLGALLFLYTGDFFLHIRFHEDSTSKELPPSWFCLPSVHVCALEAQA
Gene ID 83696
Swiss prot Q96Q05
Synonyms T1; IBP; NIBP; MRT13; TRS120; IKBKBBP; TRAPPC9
Calculated MW 129kDa
Observed MW 150kDa

Applications

Reactivity Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:200 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Mouse pancreas
Cellular location Cytoplasm, Endoplasmic reticulum, Golgi apparatus, cis-Golgi network

TRAPPC9 Rabbit pAb images

ABclonal:Western blot - TRAPPC9 Rabbit pAb (A15527)}

Western blot - TRAPPC9 Rabbit pAb (A15527)

Western blot analysis of lysates from mouse pancreas, using TRAPPC9 Rabbit pAb (A15527) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

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Related Targets

Based on existing publications, the following genes are closely related to research on TRAPPC9. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TRAPPC9. (Distance between topics and target gene indicate popularity.) TRAPPC9

* Data provided by citexs.com, for reference only.

Publishing research using A15527? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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