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TPM1 Polyclonal Antibody (A1157)

Review (1)Publication (1) Datasheet

ABclonal:Western blot - TPM1 Polyclonal Antibody (A1157)
ABclonal:Immunohistochemistry - TPM1 Polyclonal Antibody (A1157)
ABclonal:Immunohistochemistry - TPM1 Polyclonal Antibody (A1157)
ABclonal:Immunohistochemistry - TPM1 Polyclonal Antibody (A1157)
ABclonal:Immunofluorescence - TPM1 Polyclonal Antibody (A1157)
Review
ABclonal: review for TPM1 Polyclonal Antibody(A1157)

Overview

Product nameTPM1 Polyclonal Antibody
Catalog No.A1157
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human TPM1 (NP_001018008.1).
SequenceMAGSSSLEAVRRKIRSLQEQADAAEERAGTLQRELDHERKLRETAEADVASLNRRIQLVEEELDRAQERLATALQKLEEAEKAADESERGMKVIESRAQKDEEKMEIQEIQLKEAKHIAEDADRKYEEVARKLVIIESDLERAEERAELSEGKCAELEEELKTVTNNLKSLEAQAEKYSQKEDRYEEEIKVLSDKLKEAETRAEFAERSVTKLEKSIDDLEDQLYQQLEQNRRLTNELKLALNED
Gene ID7168
Swiss protP09493
SynonymsTPM1; C15orf13; CMD1Y; CMH3; HEL-S-265; HTM-alpha; LVNC9; TMSA; tropomyosin 1 (alpha)
Calculated MW26kDa/28kDa/32kDa
Observed MW36kDa
ReactivityHuman, Mouse, Rat, Zebrafish
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesA-549, BT-474, THP-1, DU145, SW480, MCF7, Mouse heart, Mouse skeletal muscle, Mouse brain
Cellular locationCytoplasm, cytoskeleton
Customer validation

WB(Zebrafish)

ABclonal:Western blot - TPM1 Polyclonal Antibody (A1157) }

Western blot - TPM1 Polyclonal Antibody (A1157)

Western blot analysis of extracts of various cell lines, using TPM1 antibody (A1157) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - TPM1 Polyclonal Antibody (A1157) }

Immunohistochemistry - TPM1 Polyclonal Antibody (A1157)

Immunohistochemistry of paraffin-embedded human lung using TPM1 antibody (A1157) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TPM1 Polyclonal Antibody (A1157) }

Immunohistochemistry - TPM1 Polyclonal Antibody (A1157)

Immunohistochemistry of paraffin-embedded human appendix using TPM1 antibody (A1157) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - TPM1 Polyclonal Antibody (A1157) }

Immunohistochemistry - TPM1 Polyclonal Antibody (A1157)

Immunohistochemistry of paraffin-embedded human uterus using TPM1 antibody (A1157) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - TPM1 Polyclonal Antibody (A1157) }

Immunofluorescence - TPM1 Polyclonal Antibody (A1157)

Immunofluorescence analysis of HeLa cells using TPM1 antibody (A1157). Blue: DAPI for nuclear staining.

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