Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

p53 Rabbit pAb (A11232)

Review (1) Publications (8) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Immunohistochemistry - p53 Rabbit pAb (A11232)

Immunohistochemistry analysis of paraffin-embedded Rat brain using p53 antibody (A11232) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - p53 Rabbit pAb (A11232)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using p53 antibody (A11232) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - p53 Rabbit pAb (A11232)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using p53 antibody (A11232) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - p53 Rabbit pAb (A11232)

Immunohistochemistry analysis of paraffin-embedded Mouse lung using p53 antibody (A11232) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - p53 Rabbit pAb (A11232)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using p53 antibody (A11232) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - p53 Rabbit pAb (A11232)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using p53 antibody (A11232) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Review
ABclonal: review for p53 Rabbit pAb(A11232)

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Overview

Product name p53 Rabbit pAb
Catalog No. A11232
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mice deficient for this gene are developmentally normal but are susceptible to spontaneous tumors. Evidence to date shows that this gene contains one promoter, in contrast to alternative promoters of the human gene, and transcribes a few of splice variants which encode different isoforms, although the biological validity or the full-length nature of some variants has not been determined.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 95-390 of mouse p53 (NP_035770.2).
Sequence PSQKTYQGNYGFHLGFLQSGTAKSVMCTYSPPLNKLFCQLAKTCPVQLWVSATPPAGSRVRAMAIYKKSQHMTEVVRRCPHHERCSDGDGLAPPQHLIRVEGNLYPEYLEDRQTFRHSVVVPYEPPEAGSEYTTIHYKYMCNSSCMGGMNRRPILTIITLEDSSGNLLGRDSFEVRVCACPGRDRRTEEENFRKKEVLCPELPPGSAKRALPTCTSASPPQKKKPLDGEYFTLKIRGRKRFEMFRELNEALELKDAHATEESGDSRAHSSYLKTKKGQSTSRHKKTMVKKVGPDSD
Gene ID 22059
Swiss prot P02340
Synonyms bbl; bfy; bhy; p44; p53; Tp53
Calculated MW 43kDa
Observed MW

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Immunohistochemistry    Immunofluorescence    
Positive samples
Cellular location Cytoplasm, Endoplasmic reticulum, Mitochondrion matrix, Nucleus, PML body
Customer validation

WB (rat cellsHomo sapiens, Homo sapiens, Rattus norvegicus, Mus musculus)

IHC (Rattus norvegicus)

IF (Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

p53 Rabbit pAb images

ABclonal:Immunohistochemistry - p53 Rabbit pAb (A11232)}

Immunohistochemistry - p53 Rabbit pAb (A11232)

Immunohistochemistry analysis of paraffin-embedded Rat brain using p53 antibody (A11232) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - p53 Rabbit pAb (A11232)}

Immunohistochemistry - p53 Rabbit pAb (A11232)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using p53 antibody (A11232) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - p53 Rabbit pAb (A11232)}

Immunohistochemistry - p53 Rabbit pAb (A11232)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using p53 antibody (A11232) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - p53 Rabbit pAb (A11232)}

Immunohistochemistry - p53 Rabbit pAb (A11232)

Immunohistochemistry analysis of paraffin-embedded Mouse lung using p53 antibody (A11232) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - p53 Rabbit pAb (A11232)}

Immunohistochemistry - p53 Rabbit pAb (A11232)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using p53 antibody (A11232) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - p53 Rabbit pAb (A11232)}

Immunohistochemistry - p53 Rabbit pAb (A11232)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using p53 antibody (A11232) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on Trp53. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to Trp53. (Distance between topics and target gene indicate popularity.) Trp53

* Data provided by citexs.com, for reference only.

Publishing research using A11232? Please let us know so that we can cite the reference in this datasheet.

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Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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