Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

TNFR1/TNFRSF1A Rabbit pAb (A1540)

Publications (7) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - TNFR1/TNFRSF1A Rabbit pAb (A1540)

Western blot analysis of extracts of various cell lines, using TNFR1/TNFRSF1A antibody (A1540) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - TNFR1/TNFRSF1A Rabbit pAb (A1540)

Immunohistochemistry analysis of paraffin-embedded rat spleen using TNFR1/TNFRSF1A Rabbit pAb (A1540) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - TNFR1/TNFRSF1A Rabbit pAb (A1540)

Immunofluorescence analysis of BALB-3T3 cells using TNFR1/TNFRSF1A Rabbit pAb (A1540) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Overview

Product name TNFR1/TNFRSF1A Rabbit pAb
Catalog No. A1540
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the TNF receptor superfamily of proteins. The encoded receptor is found in membrane-bound and soluble forms that interact with membrane-bound and soluble forms, respectively, of its ligand, tumor necrosis factor alpha. Binding of membrane-bound tumor necrosis factor alpha to the membrane-bound receptor induces receptor trimerization and activation, which plays a role in cell survival, apoptosis, and inflammation. Proteolytic processing of the encoded receptor results in release of the soluble form of the receptor, which can interact with free tumor necrosis factor alpha to inhibit inflammation. Mutations in this gene underlie tumor necrosis factor receptor-associated periodic syndrome (TRAPS), characterized by fever, abdominal pain and other features. Mutations in this gene may also be associated with multiple sclerosis in human patients.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 345-455 of human TNFR1/TNFRSF1A (NP_001056.1).
Sequence AHKPQSLDTDDPATLYAVVENVPPLRWKEFVRRLGLSDHEIDRLELQNGRCLREAQYSMLATWRRRTPRREATLELLGRVLRDMDLLGCLEDIEEALCGPAALPPAPSLLR
Gene ID 7132
Swiss prot P19438
Synonyms FPF; p55; p60; TBP1; TNF-R; TNFAR; TNFR1; p55-R; CD120a; TNFR55; TNFR60; TNF-R-I; TNF-R55; TNFR1/TNFRSF1A
Calculated MW 50kDa
Observed MW 55kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples A-549, C6
Cellular location Cell membrane, Golgi apparatus membrane, Secreted, Secreted, Single-pass type I membrane protein
Customer validation

WB (Mus musculus, Homo sapiens, Marine Streptomyces sp, Rattus norvegicus)

IF (Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

TNFR1/TNFRSF1A Rabbit pAb images

ABclonal:Western blot - TNFR1/TNFRSF1A Rabbit pAb (A1540)}

Western blot - TNFR1/TNFRSF1A Rabbit pAb (A1540)

Western blot analysis of extracts of various cell lines, using TNFR1/TNFRSF1A antibody (A1540) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - TNFR1/TNFRSF1A Rabbit pAb (A1540)}

Immunohistochemistry - TNFR1/TNFRSF1A Rabbit pAb (A1540)

Immunohistochemistry analysis of paraffin-embedded rat spleen using TNFR1/TNFRSF1A Rabbit pAb (A1540) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - TNFR1/TNFRSF1A Rabbit pAb (A1540)}

Immunofluorescence - TNFR1/TNFRSF1A Rabbit pAb (A1540)

Immunofluorescence analysis of BALB-3T3 cells using TNFR1/TNFRSF1A Rabbit pAb (A1540) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on TNFRSF1A. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TNFRSF1A. (Distance between topics and target gene indicate popularity.) TNFRSF1A

* Data provided by citexs.com, for reference only.

Publishing research using A1540? Please let us know so that we can cite the reference in this datasheet.

ELISA Kits (2)

Proteins (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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