Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

TICAM2 Rabbit pAb (A8292)

Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - TICAM2 Rabbit pAb (A8292)

Western blot analysis of extracts of various cell lines, using TICAM2 antibody (A8292) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Overview

Product name TICAM2 Rabbit pAb
Catalog No. A8292
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Predicted to enable phospholipid binding activity. Involved in several processes, including TRAM-dependent toll-like receptor 4 signaling pathway; regulation of chemokine (C-C motif) ligand 5 production; and regulation of toll-like receptor 4 signaling pathway. Located in endoplasmic reticulum; endosome; and phagocytic cup.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-235 of human TICAM2 (NP_067681.1).
Sequence MGIGKSKINSCPLSLSWGKRHSVDTSPGYHESDSKKSEDLSLCNVAEHSNTTEGPTGKQEGAQSVEEMFEEEAEEEVFLKFVILHAEDDTDEALRVQNLLQDDFGIKPGIIFAEMPCGRQHLQNLDDAVNGSAWTILLLTENFLRDTWCNFQFYTSLMNSVNRQHKYNSVIPMRPLNNPLPRERTPFALQTINALEEESRGFPTQVERIFQESVYKTQQTIWKETRNMVQRQFIA
Gene ID 353376
Swiss prot Q86XR7
Synonyms TIRP; TRAM; TIRAP3; MyD88-4; TICAM-2; TICAM2
Calculated MW 27kDa
Observed MW 37kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples 22Rv1, 293T, Jurkat, Raji, SW480
Cellular location Cell membrane, Cytoplasm, Early endosome membrane, Endoplasmic reticulum, Golgi apparatus, Late endosome membrane, Late endosome membrane

TICAM2 Rabbit pAb images

ABclonal:Western blot - TICAM2 Rabbit pAb (A8292)}

Western blot - TICAM2 Rabbit pAb (A8292)

Western blot analysis of extracts of various cell lines, using TICAM2 antibody (A8292) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Related Targets

Based on existing publications, the following genes are closely related to research on TICAM2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TICAM2. (Distance between topics and target gene indicate popularity.) TICAM2

* Data provided by citexs.com, for reference only.

Publishing research using A8292? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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