Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

THAP3 Rabbit pAb (A12899)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Mouse, Rat

ABclonal:Western blot - THAP3 Rabbit pAb (A12899)

Western blot analysis of various lysates using THAP3 Rabbit pAb (A12899) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Overview

Product name THAP3 Rabbit pAb
Catalog No. A12899
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Predicted to enable DNA binding activity and metal ion binding activity. Involved in positive regulation of transcription by RNA polymerase II.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-239 of human THAP3 (NP_001182682.1).
Sequence MPKSCAARQCCNRYSSRRKQLTFHRFPFSRPELLKEWVLNIGRGNFKPKQHTVICSEHFRPECFSAFGNRKNLKHNAVPTVFAFQDPTQQVRENTDPASERGNASSSQKEKVLPEAGAGEDSPGRNMDTALEELQLPPNAEGHVKQVSPRRPQATEAVGRPTGPAGLRRTPNKQPSDHSYALLDLDSLKKKLFLTLKENEKLRKRLQAQRLVMRRMSSRLRACKGHQGLQARLGPEQQS
Gene ID 90326
Swiss prot Q8WTV1
Synonyms THAP3
Calculated MW 27kDa
Observed MW 27kDa

Applications

Reactivity Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Mouse kidney, Rat liver
Cellular location

Documents

Certificate of Compliance

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Lot number

THAP3 Rabbit pAb images

ABclonal:Western blot - THAP3 Rabbit pAb (A12899)}

Western blot - THAP3 Rabbit pAb (A12899)

Western blot analysis of various lysates using THAP3 Rabbit pAb (A12899) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on THAP3. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to THAP3. (Distance between topics and target gene indicate popularity.) THAP3

* Data provided by citexs.com, for reference only.

Publishing research using A12899? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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