Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Transferrin Rabbit pAb (A1448)

Publications (5) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Transferrin Rabbit pAb (A1448)

Western blot analysis of extracts of various cell lines, using Transferrin antibody (A1448) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - Transferrin Rabbit pAb (A1448)

Western blot analysis of extracts of various cell lines, using Transferrin antibody (A1448) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - Transferrin Rabbit pAb (A1448)

Immunofluorescence analysis of HepG2 cells using Transferrin Rabbit pAb (A1448) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - Transferrin Rabbit pAb (A1448)

Immunofluorescence analysis of human liver cells using Transferrin Rabbit pAb (A1448) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

You may also interested in:

Overview

Product name Transferrin Rabbit pAb
Catalog No. A1448
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 355-698 of human Transferrin (NP_001054.2).
Sequence TDECKPVKWCALSHHERLKCDEWSVNSVGKIECVSAETTEDCIAKIMNGEADAMSLDGGFVYIAGKCGLVPVLAENYNKSDNCEDTPEAGYFAIAVVKKSASDLTWDNLKGKKSCHTAVGRTAGWNIPMGLLYNKINHCRFDEFFSEGCAPGSKKDSSLCKLCMGSGLNLCEPNNKEGYYGYTGAFRCLVEKGDVAFVKHQTVPQNTGGKNPDPWAKNLNEKDYELLCLDGTRKPVEEYANCHLARAPNHAVVTRKDKEACVHKILRQQQHLFGSNVTDCSGNFCLFRSETKDLLFRDDTVCLAKLHDRNTYEKYLGEEYVKAVGNLRKCSTSSLLEACTFRRP
Gene ID 7018
Swiss prot P02787
Synonyms TFQTL1; PRO1557; PRO2086; HEL-S-71p; Transferrin
Calculated MW 77kDa
Observed MW 77kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HepG2, HUH-7, Human plasma, Mouse plasma, Rat plasma
Cellular location Secreted
Customer validation

WB (Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Transferrin Rabbit pAb images

ABclonal:Western blot - Transferrin Rabbit pAb (A1448)}

Western blot - Transferrin Rabbit pAb (A1448)

Western blot analysis of extracts of various cell lines, using Transferrin antibody (A1448) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - Transferrin Rabbit pAb (A1448)}

Western blot - Transferrin Rabbit pAb (A1448)

Western blot analysis of extracts of various cell lines, using Transferrin antibody (A1448) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - Transferrin Rabbit pAb (A1448)}

Immunofluorescence - Transferrin Rabbit pAb (A1448)

Immunofluorescence analysis of HepG2 cells using Transferrin Rabbit pAb (A1448) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - Transferrin Rabbit pAb (A1448)}

Immunofluorescence - Transferrin Rabbit pAb (A1448)

Immunofluorescence analysis of human liver cells using Transferrin Rabbit pAb (A1448) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Inquire About This Product

Submit your question about A1448 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Related Targets

Based on existing publications, the following genes are closely related to research on TF. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TF. (Distance between topics and target gene indicate popularity.) TF

* Data provided by citexs.com, for reference only.

Publishing research using A1448? Please let us know so that we can cite the reference in this datasheet.

Antibodies (1)

Proteins (1)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order