Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

TEK Rabbit pAb (A7222)

Publications (5) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - TEK Rabbit pAb (A7222)

Western blot analysis of extracts of various cell lines, using TEK antibody (A7222) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Overview

Product name TEK Rabbit pAb
Catalog No. A7222
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a receptor that belongs to the protein tyrosine kinase Tie2 family. The encoded protein possesses a unique extracellular region that contains two immunoglobulin-like domains, three epidermal growth factor (EGF)-like domains and three fibronectin type III repeats. The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. Mutations in this gene are associated with inherited venous malformations of the skin and mucous membranes. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 500-750 of human TEK (NP_000450.2).
Sequence EIVTLNYLEPRTEYELCVQLVRRGEGGEGHPGPVRRFTTASIGLPPPRGLNLLPKSQTTLNLTWQPIFPSSEDDFYVEVERRSVQKSDQQNIKVPGNLTSVLLNNLHPREQYVVRARVNTKAQGEWSEDLTAWTLSDILPPQPENIKISNITHSSAVISWTILDGYSISSITIRYKVQGKNEDQHVDVKIKNATITQYQLKGLEPETAYQVDIFAENNIGSSNPAFSHELVTLPESQAPADLGGGKMLLIA
Gene ID 7010
Swiss prot Q02763
Synonyms TIE2; VMCM; GLC3E; TIE-2; VMCM1; CD202B; TEK
Calculated MW 126kDa
Observed MW 126kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples ECV-304, HUVEC
Cellular location Cell junction, Cell membrane, Cytoplasm, Secreted, Single-pass type I membrane protein, cytoskeleton, focal adhesion
Customer validation

WB (Rattus norvegicus)

IHC (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

TEK Rabbit pAb images

ABclonal:Western blot - TEK Rabbit pAb (A7222)}

Western blot - TEK Rabbit pAb (A7222)

Western blot analysis of extracts of various cell lines, using TEK antibody (A7222) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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Related Targets

Based on existing publications, the following genes are closely related to research on TEK. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TEK. (Distance between topics and target gene indicate popularity.) TEK

* Data provided by citexs.com, for reference only.

Publishing research using A7222? Please let us know so that we can cite the reference in this datasheet.

Antibodies (1)

Proteins (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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