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TEAD2 Polyclonal Antibody (A15594)

Publications (2) Datasheet

ABclonal:Western blot - TEAD2 Polyclonal Antibody (A15594)

Overview

Product nameTEAD2 Polyclonal Antibody
Catalog No.A15594
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant protein of human TEAD2
SequenceEmail for sequence
Gene ID8463
Swiss protQ15562
SynonymsTEAD2; ETF; TEAD-2; TEF-4; TEF4
Calculated MW35kDa/49kDa
Observed MW70KDa
ReactivityMouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesMouse lung, Mouse testis, Rat lung, Rat testis
Cellular location
Customer validation

WB(Mouse)

IP(Mouse)

IF(Mouse)

CHIP(Mouse)

ABclonal:Western blot - TEAD2 Polyclonal Antibody (A15594) }

Western blot - TEAD2 Polyclonal Antibody (A15594)

Western blot analysis of extracts of various cell lines, using TEAD2 antibody (A15594) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 300S.

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