Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

TBX20 Rabbit pAb (A15994)

Publication (1) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Rat

ABclonal:Western blot - TBX20 Rabbit pAb (A15994)

Western blot analysis of lysates from Rat heart, using TBX20 Rabbit pAb (A15994) at 1:900 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - TBX20 Rabbit pAb (A15994)

Immunohistochemistry analysis of TBX20 in paraffin-embedded human placenta using TBX20 Rabbit pAb (A15994) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Overview

Product name TBX20 Rabbit pAb
Catalog No. A15994
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a T-box family member. The T-box family members share a common DNA binding domain, termed the T-box, and they are transcription factors involved in the regulation of developmental processes. This gene is essential for heart development. Mutations in this gene are associated with diverse cardiac pathologies, including defects in septation, valvulogenesis and cardiomyopathy. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human TBX20 (NP_001071121.1).
Sequence MEFTASPKPQLSSRANAFSIAALMSSGGSKEKEATENTIKPLEQFVEKSSCAQPLGELTSLDAHGEFGGGSGSSPSSSSLCTEPLIPTTPIIPSEEMAKIACSLETKELWDKFHELGTEMIITKSGRRMFPTIRVSFSGVDPEAKYIVLMDIVPVDNKRYRYAYHRSSWLVAGKADPPLPARLYVHPDSPFTGEQLLKQMVSFEKVKLTNNELDQHGHIILNSMHKYQPRVHIIKKKDHTASLLNLKSEEFRTFIFPETVFTAVTAYQNQLITKLKIDSNPFAKGFRDSS
Gene ID 57057
Swiss prot Q9UMR3
Synonyms ASD4; TBX20
Calculated MW 49kDa
Observed MW 49kDa

Applications

Reactivity Human, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples Rat heart
Cellular location cytoplasm, nucleus
Customer validation

WB (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

TBX20 Rabbit pAb images

ABclonal:Western blot - TBX20 Rabbit pAb (A15994)}

Western blot - TBX20 Rabbit pAb (A15994)

Western blot analysis of lysates from Rat heart, using TBX20 Rabbit pAb (A15994) at 1:900 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - TBX20 Rabbit pAb (A15994)}

Immunohistochemistry - TBX20 Rabbit pAb (A15994)

Immunohistochemistry analysis of TBX20 in paraffin-embedded human placenta using TBX20 Rabbit pAb (A15994) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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Related Targets

Based on existing publications, the following genes are closely related to research on TBX20. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to TBX20. (Distance between topics and target gene indicate popularity.) TBX20

* Data provided by citexs.com, for reference only.

Publishing research using A15994? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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