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SULT1A3 Polyclonal Antibody (A7932)

Datasheet

ABclonal:Western blot - SULT1A3 Polyclonal Antibody (A7932)

Overview

Product nameSULT1A3 Polyclonal Antibody
Catalog No.A7932
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. This gene encodes a phenol sulfotransferase with thermolabile enzyme activity. Four sulfotransferase genes are located on the p arm of chromosome 16; this gene and SULT1A4 arose from a segmental duplication. This gene is the most centromeric of the four sulfotransferase genes. Read-through transcription exists between this gene and the upstream SLX1A (SLX1 structure-specific endonuclease subunit homolog A) gene that encodes a protein containing GIY-YIG domains.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-295 of human SULT1A3 (NP_808220.1).
SequenceMELIQDTSRPPLEYVKGVPLIKYFAEALGPLQSFQARPDDLLINTYPKSGTTWVSQILDMIYQGGDLEKCNRAPIYVRVPFLEVNDPGEPSGLETLKDTPPPRLIKSHLPLALLPQTLLDQKVKVVYVARNPKDVAVSYYHFHRMEKAHPEPGTWDSFLEKFMAGEVSYGSWYQHVQEWWELSRTHPVLYLFYEDMKENPKREIQKILEFVGRSLPEETMDFMVQHTSFKEMKKNPMTNYTTVPQELMDHSISPFMRKGMAGDWKTTFTVAQNERFDADYAEKMAGCSLSFRSEL
Gene ID6818
Swiss protP0DMM9
SynonymsSULT1A3; HAST; HAST3; M-PST; ST1A3; ST1A3/ST1A4; ST1A5; STM; TL-PST; sulfotransferase 1A3
Calculated MW34kDa
Observed MW34kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesMCF-7, HeLa, Mouse liver, Rat liver
Cellular location
ABclonal:Western blot - SULT1A3 Polyclonal Antibody (A7932) }

Western blot - SULT1A3 Polyclonal Antibody (A7932)

Western blot analysis of extracts of various cell lines, using SULT1A3 antibody (A7932) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

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