Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

SUFU Rabbit pAb (A13429)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - SUFU Rabbit pAb (A13429)

Western blot analysis of lysates from Mouse testis using SUFU Rabbit pAb(A13429) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - SUFU Rabbit pAb (A13429)

Immunohistochemistry analysis of SUFU in paraffin-embedded human breast cancer using SUFU Rabbit pAb (A13429) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SUFU Rabbit pAb (A13429)

Immunohistochemistry analysis of SUFU in paraffin-embedded human esophageal cancer using SUFU Rabbit pAb (A13429) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - SUFU Rabbit pAb (A13429)

Immunofluorescence analysis of MCF-7 cells using SUFU Rabbit pAb (A13429). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name SUFU Rabbit pAb
Catalog No. A13429
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The Hedgehog signaling pathway plays an important role in early human development. The pathway is a signaling cascade that plays a role in pattern formation and cellular proliferation during development. This gene encodes a negative regulator of the hedgehog signaling pathway. Defects in this gene are a cause of medulloblastoma. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 325-484 of human SUFU (NP_057253.2).
Sequence PPINPQRQNGLAHDRAPSRKDSLESDSSTAIIPHELIRTRQLESVHLKFNQESGALIPLCLRGRLLHGRHFTYKSITGDMAITFVSTGVEGAFATEEHPYAAHGPWLQILLTEEFVEKMLEDLEDLTSPEEFKLPKEYSWPEKKLKVSILPDVVFDSPLH
Gene ID 51684
Swiss prot Q9UMX1
Synonyms SUFUH; JBTS32; SUFUXL; PRO1280; SUFU
Calculated MW 54kDa
Observed MW 54kDa/

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples C6, PC-12, Mouse testis
Cellular location Cytoplasm, Nucleus

Documents

Certificate of Compliance

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Lot number

Research Area

SUFU Rabbit pAb images

ABclonal:Western blot - SUFU Rabbit pAb (A13429)}

Western blot - SUFU Rabbit pAb (A13429)

Western blot analysis of lysates from Mouse testis using SUFU Rabbit pAb(A13429) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - SUFU Rabbit pAb (A13429)}

Immunohistochemistry - SUFU Rabbit pAb (A13429)

Immunohistochemistry analysis of SUFU in paraffin-embedded human breast cancer using SUFU Rabbit pAb (A13429) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - SUFU Rabbit pAb (A13429)}

Immunohistochemistry - SUFU Rabbit pAb (A13429)

Immunohistochemistry analysis of SUFU in paraffin-embedded human esophageal cancer using SUFU Rabbit pAb (A13429) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - SUFU Rabbit pAb (A13429)}

Immunofluorescence - SUFU Rabbit pAb (A13429)

Immunofluorescence analysis of MCF-7 cells using SUFU Rabbit pAb (A13429). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on SUFU. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to SUFU. (Distance between topics and target gene indicate popularity.) SUFU

* Data provided by citexs.com, for reference only.

Publishing research using A13429? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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