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STAT1 Polyclonal Antibody (A12075)

Reviews (2) Datasheet

ABclonal:Western blot - STAT1 Polyclonal Antibody (A12075)
ABclonal:Immunohistochemistry - STAT1 Polyclonal Antibody (A12075)
ABclonal:Immunohistochemistry - STAT1 Polyclonal Antibody (A12075)
ABclonal:Immunofluorescence - STAT1 Polyclonal Antibody (A12075)
ABclonal:Immunoprecipitation - STAT1 Polyclonal Antibody (A12075)
Review
ABclonal: review for STAT1 Polyclonal Antibody(A12075)
Review
ABclonal: review for STAT1 Polyclonal Antibody(A12075)

Overview

Product nameSTAT1 Polyclonal Antibody
Catalog No.A12075
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 650-750 of human STAT1 (NP_009330.1).
SequenceNYKVMAAENIPENPLKYLYPNIDKDHAFGKYYSRPKEAPEPMELDGPKGTGYIKTELISVSEVHPSRLQTTDNLLPMSPEEFDEVSRIVGSVEFDSMMNTV
Gene ID6772
Swiss protP42224
SynonymsSTAT1; CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91; signal transducer and activator of transcription 1
Calculated MW83kDa/87kDa
Observed MW91kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa
Cellular locationCytoplasm, Nucleus
Customer validation

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ABclonal:Western blot - STAT1 Polyclonal Antibody (A12075) }

Western blot - STAT1 Polyclonal Antibody (A12075)

Western blot analysis of extracts of HeLa cells, using STAT1 antibody (A12075) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - STAT1 Polyclonal Antibody (A12075) }

Immunohistochemistry - STAT1 Polyclonal Antibody (A12075)

Immunohistochemistry of paraffin-embedded human mammary cancer using STAT1 antibody (A12075) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - STAT1 Polyclonal Antibody (A12075) }

Immunohistochemistry - STAT1 Polyclonal Antibody (A12075)

Immunohistochemistry of paraffin-embedded human gastric cancer using STAT1 antibody (A12075) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - STAT1 Polyclonal Antibody (A12075) }

Immunofluorescence - STAT1 Polyclonal Antibody (A12075)

Immunofluorescence analysis of U2OS cells using STAT1 antibody (A12075) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - STAT1 Polyclonal Antibody (A12075) }

Immunoprecipitation - STAT1 Polyclonal Antibody (A12075)

Immunoprecipitation analysis of 100ug extracts of HeLa cells using 3ug STAT1 antibody (A12075). Western blot was performed from the immunoprecipitate using STAT1 antibody (A12075) at a dilition of 1:1000.

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