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SPTAN1 Polyclonal Antibody (A0160)

Publication (1) Datasheet

ABclonal:Western blot - SPTAN1 Polyclonal Antibody (A0160)
ABclonal:Immunofluorescence - SPTAN1 Polyclonal Antibody (A0160)
ABclonal:Immunofluorescence - SPTAN1 Polyclonal Antibody (A0160)

Overview

Product nameSPTAN1 Polyclonal Antibody
Catalog No.A0160
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. This gene encodes an alpha spectrin that is specifically expressed in nonerythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in this gene are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 950-1130 of human SPTAN1 (NP_001123910.1).
SequenceREQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQ
Gene ID6709
Swiss protQ13813
SynonymsSPTAN1; EIEE5; NEAS; SPTA2; spectrin alpha, non-erythrocytic 1
Calculated MW282kDa/284kDa/285kDa
Observed MW285kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesU-87MG, Jurkat, A-549, NIH/3T3, Mouse kidney, Rat spinal cord
Cellular locationCytoplasm, cell cortex, cytoskeleton
Customer validation

WB(Mouse)

ABclonal:Western blot - SPTAN1 Polyclonal Antibody (A0160) }

Western blot - SPTAN1 Polyclonal Antibody (A0160)

Western blot analysis of extracts of various cell lines, using SPTAN1 antibody (A0160) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - SPTAN1 Polyclonal Antibody (A0160) }

Immunofluorescence - SPTAN1 Polyclonal Antibody (A0160)

Immunofluorescence analysis of U-2 OS cells using SPTAN1 antibody (A0160) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SPTAN1 Polyclonal Antibody (A0160) }

Immunofluorescence - SPTAN1 Polyclonal Antibody (A0160)

Immunofluorescence analysis of U-2 OS cells using SPTAN1 antibody (A0160) at dilution of 1:100. Blue: DAPI for nuclear staining.

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