Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

Alpha Fodrin Rabbit pAb (A0160)

Publications (2) Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Alpha Fodrin Rabbit pAb (A0160)

Western blot analysis of various lysates using Alpha Fodrin Rabbit pAb (A0160) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - Alpha Fodrin Rabbit pAb (A0160)

Immunohistochemistry analysis of Alpha Fodrin in paraffin-embedded rat liver using Alpha Fodrin Rabbit pAb (A0160) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Alpha Fodrin Rabbit pAb (A0160)

Immunohistochemistry analysis of Alpha Fodrin in paraffin-embedded human stomach using Alpha Fodrin Rabbit pAb (A0160) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - Alpha Fodrin Rabbit pAb (A0160)

Immunohistochemistry analysis of Alpha Fodrin in paraffin-embedded mouse kidney using Alpha Fodrin Rabbit pAb (A0160) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - Alpha Fodrin Rabbit pAb (A0160)

Immunofluorescence analysis of U-2 OS cells using Alpha Fodrin Rabbit pAb (A0160) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name Alpha Fodrin Rabbit pAb
Catalog No. A0160
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Spectrins are a family of filamentous cytoskeletal proteins that function as essential scaffold proteins that stabilize the plasma membrane and organize intracellular organelles. Spectrins are composed of alpha and beta dimers that associate to form tetramers linked in a head-to-head arrangement. This gene encodes an alpha spectrin that is specifically expressed in nonerythrocytic cells. The encoded protein has been implicated in other cellular functions including DNA repair and cell cycle regulation. Mutations in this gene are the cause of early infantile epileptic encephalopathy-5. Alternate splicing results in multiple transcript variants.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 950-1130 of human Alpha Fodrin (NP_001123910.1).
Sequence REQAQSCRQQVAPTDDETGKELVLALYDYQEKSPREVTMKKGDILTLLNSTNKDWWKVEVNDRQGFVPAAYVKKLDPAQSASRENLLEEQGSIALRQEQIDNQTRITKEAGSVSLRMKQVEELYHSLLELGEKRKGMLEKSCKKFMLFREANELQQWINEKEAALTSEEVGADLEQVEVLQ
Gene ID 6709
Swiss prot Q13813
Synonyms DEE5; NEAS; EIEE5; SPTA2; Alpha Fodrin
Calculated MW 285kDa
Observed MW 285kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples U-87MG, Jurkat, A-549, NIH/3T3, Mouse kidney, Rat spinal cord
Cellular location Cytoplasm, cell cortex, cytoskeleton
Customer validation

WB (Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

Alpha Fodrin Rabbit pAb images

ABclonal:Western blot - Alpha Fodrin Rabbit pAb (A0160)}

Western blot - Alpha Fodrin Rabbit pAb (A0160)

Western blot analysis of various lysates using Alpha Fodrin Rabbit pAb (A0160) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - Alpha Fodrin Rabbit pAb (A0160)}

Immunohistochemistry - Alpha Fodrin Rabbit pAb (A0160)

Immunohistochemistry analysis of Alpha Fodrin in paraffin-embedded rat liver using Alpha Fodrin Rabbit pAb (A0160) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Alpha Fodrin Rabbit pAb (A0160)}

Immunohistochemistry - Alpha Fodrin Rabbit pAb (A0160)

Immunohistochemistry analysis of Alpha Fodrin in paraffin-embedded human stomach using Alpha Fodrin Rabbit pAb (A0160) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - Alpha Fodrin Rabbit pAb (A0160)}

Immunohistochemistry - Alpha Fodrin Rabbit pAb (A0160)

Immunohistochemistry analysis of Alpha Fodrin in paraffin-embedded mouse kidney using Alpha Fodrin Rabbit pAb (A0160) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - Alpha Fodrin Rabbit pAb (A0160)}

Immunofluorescence - Alpha Fodrin Rabbit pAb (A0160)

Immunofluorescence analysis of U-2 OS cells using Alpha Fodrin Rabbit pAb (A0160) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on SPTAN1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to SPTAN1. (Distance between topics and target gene indicate popularity.) SPTAN1

* Data provided by citexs.com, for reference only.

Publishing research using A0160? Please let us know so that we can cite the reference in this datasheet.

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Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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