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SPTA1 Polyclonal Antibody (A12355)

Publication (2) Datasheet

ABclonal:Western blot - SPTA1 Polyclonal Antibody (A12355)
ABclonal:Immunohistochemistry - SPTA1 Polyclonal Antibody (A12355)
ABclonal:Immunohistochemistry - SPTA1 Polyclonal Antibody (A12355)
ABclonal:Immunohistochemistry - SPTA1 Polyclonal Antibody (A12355)
ABclonal:Immunofluorescence - SPTA1 Polyclonal Antibody (A12355)
ABclonal:Immunofluorescence - SPTA1 Polyclonal Antibody (A12355)

Overview

Product nameSPTA1 Polyclonal Antibody
Catalog No.A12355
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is a tetramer made up of alpha-beta dimers linked in a head-to-head arrangement. This gene is one member of a family of alpha-spectrin genes. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms weaker tetramer interactions than non-erythrocytic alpha spectrin, which may increase the plasma membrane elasticity and deformability of red blood cells. Mutations in this gene result in a variety of hereditary red blood cell disorders, including elliptocytosis type 2, pyropoikilocytosis, and spherocytic hemolytic anemia.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 940-1160 of human SPTA1 (NP_003117.2).
SequenceKHEAFLLDLNSFGDSMKALRNQANACQQQQAAPVEGVAGEQRVMALYDFQARSPREVTMKKGDVLTLLSSINKDWWKVEAADHQGIVPAVYVRRLAHDEFPMLPQRRREEPGNITQRQEQIENQYRSLLDRAEERRRRLLQRYNEFLLAYEAGDMLEWIQEKKAENTGVELDDVWELQKKFDEFQKDLNTNEPRLRDINKVADDLLFEGLLTPEGAQIRQE
Gene ID6708
Swiss protP02549
SynonymsSPTA1; EL2; HPP; HS3; SPH3; SPTA
Calculated MW279kDa/280kDa
Observed MW300kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples239T, LO2, A-549, Mouse kidney, Mouse liver, Mouse lung, Rat heart
Cellular locationCytoplasm, cell cortex, cytoskeleton
Customer validation

IP(Mouse)

IF(Mouse)

ABclonal:Western blot - SPTA1 Polyclonal Antibody (A12355) }

Western blot - SPTA1 Polyclonal Antibody (A12355)

Western blot analysis of extracts of various cell lines, using SPTA1 antibody (A12355) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - SPTA1 Polyclonal Antibody (A12355) }

Immunohistochemistry - SPTA1 Polyclonal Antibody (A12355)

Immunohistochemistry of paraffin-embedded human tonsil using SPTA1 antibody (A12355) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - SPTA1 Polyclonal Antibody (A12355) }

Immunohistochemistry - SPTA1 Polyclonal Antibody (A12355)

Immunohistochemistry of paraffin-embedded rat spleen using SPTA1 antibody (A12355) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - SPTA1 Polyclonal Antibody (A12355) }

Immunohistochemistry - SPTA1 Polyclonal Antibody (A12355)

Immunohistochemistry of paraffin-embedded mouse liver using SPTA1 antibody (A12355) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - SPTA1 Polyclonal Antibody (A12355) }

Immunofluorescence - SPTA1 Polyclonal Antibody (A12355)

Immunofluorescence analysis of rat bone marrow cells using SPTA1 antibody (A12355) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - SPTA1 Polyclonal Antibody (A12355) }

Immunofluorescence - SPTA1 Polyclonal Antibody (A12355)

Immunofluorescence analysis of mouse lung cells using SPTA1 antibody (A12355) at dilution of 1:100. Blue: DAPI for nuclear staining.

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