Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

SNX6 Rabbit pAb (A10039)

Publication (1) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - SNX6 Rabbit pAb (A10039)

Western blot analysis of extracts of various cell lines, using SNX6 antibody (A10039) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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Overview

Product name SNX6 Rabbit pAb
Catalog No. A10039
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein associates with the long isoform of the leptin receptor, the transforming growth factor-beta family of receptor serine-threonine kinases, and with receptor tyrosine kinases for platelet-derived growth factor, insulin, and epidermal growth factor. This protein may form oligomeric complexes with family member proteins through interactions of both the PX domain and the coiled coil regions of the molecules. Translocation of this protein from the cytoplasm to the nucleus occurs after binding to proviral integration site 1 protein. This gene results in two transcripts encoding two distinct isoforms.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-290 of human SNX6 (NP_067072.3).
Sequence MTKEEFTKMKQELEAEYLAIFKKTVAMHEVFLCRVAAHPILRRDLNFHVFLEYNQDLSVRGKNKKEKLEDFFKNMVKSADGVIVSGVKDVDDFFEHERTFLLEYHNRVKDASAKSDRMTRSHKSAADDYNRIGSSLYALGTQDSTDICKFFLKVSELFDKTRKIEARVSADEDLKLSDLLKYYLRESQAAKDLLYRRSRSLVDYENANKALDKARAKNKDVLQAETSQQLCCQKFEKISESAKQELIDFKTRRVAAFRKNLVELAELELKHAKGNLQLLQNCLAVLNGDT
Gene ID 58533
Swiss prot Q9UNH7
Synonyms TFAF2; MSTP010; SNX6
Calculated MW 47kDa
Observed MW 46kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HepG2, DU145, BT-474, Mouse kidney, Mouse lung, Mouse thymus, Mouse pancreas, Rat kidney
Cellular location Cytoplasm, Cytoplasmic side, Cytoplasmic vesicle, Early endosome, Early endosome membrane, Nucleus, Peripheral membrane protein
Customer validation

WB (Homo sapiens)

Research Area

SNX6 Rabbit pAb images

ABclonal:Western blot - SNX6 Rabbit pAb (A10039)}

Western blot - SNX6 Rabbit pAb (A10039)

Western blot analysis of extracts of various cell lines, using SNX6 antibody (A10039) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

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Related Targets

Based on existing publications, the following genes are closely related to research on SNX6. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to SNX6. (Distance between topics and target gene indicate popularity.) SNX6

* Data provided by citexs.com, for reference only.

Publishing research using A10039? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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