Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

SIRT1 Rabbit pAb (A0230)

Review (1) Publications (13) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - SIRT1 Rabbit pAb (A0230)

Western blot analysis of lysates from Mouse testis, using SIRT1 Rabbit pAb (A0230) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - SIRT1 Rabbit pAb (A0230)

Immunofluorescence analysis of NIH-3T3 cells using SIRT1 Polyclonal Antibody (A0230) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for SIRT1 Rabbit pAb(A0230)

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Overview

Product name SIRT1 Rabbit pAb
Catalog No. A0230
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 201-300 of human SIRT1 (NP_036370.2).
Sequence SEPPKRKKRKDINTIEDA
Gene ID 23411
Swiss prot Q96EB6
Synonyms SIR2; SIR2L1; SIR2alpha; SIRT1
Calculated MW 82kDa
Observed MW 100-120kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples Mouse testis
Cellular location Cytoplasm, Mitochondrion, Nucleus, Nucleus, PML body
Customer validation

WB (HeLaHomo sapiens, Mus musculus, Homo sapiens, Rattus norvegicus)

IF (Sus scrofa, Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

SIRT1 Rabbit pAb images

ABclonal:Western blot - SIRT1 Rabbit pAb (A0230)}

Western blot - SIRT1 Rabbit pAb (A0230)

Western blot analysis of lysates from Mouse testis, using SIRT1 Rabbit pAb (A0230) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - SIRT1 Rabbit pAb (A0230)}

Immunofluorescence - SIRT1 Rabbit pAb (A0230)

Immunofluorescence analysis of NIH-3T3 cells using SIRT1 Polyclonal Antibody (A0230) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on SIRT1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to SIRT1. (Distance between topics and target gene indicate popularity.) SIRT1

* Data provided by citexs.com, for reference only.

Publishing research using A0230? Please let us know so that we can cite the reference in this datasheet.

Antibodies (5)

ELISA Kits (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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