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SF3B6 Polyclonal Antibody (A13097)

Datasheet

ABclonal:Western blot - SF3B6 Polyclonal Antibody (A13097)

Overview

Product nameSF3B6 Polyclonal Antibody
Catalog No.A13097
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human SF3B6 (NP_057131.1).
SequenceMAMQAAKRANIRLPPEVNRILYIRNLPYKITAEEMYDIFGKYGPIRQIRVGNTPETRGTAYVVYEDIFDAKNACDHLSGFNVCNRYLVVLYYNANRAFQKMDTKKKEEQLKLLKEKYGINTDPPK
Gene ID51639
Swiss protQ9Y3B4
SynonymsSF3B6; CGI-110; HSPC175; Ht006; P14; SAP14; SAP14a; SF3B14; SF3B14a; splicing factor 3B subunit 6
Calculated MW14kDa
Observed MW14kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa, K-562, Mouse spleen
Cellular locationNucleus
ABclonal:Western blot - SF3B6 Polyclonal Antibody (A13097) }

Western blot - SF3B6 Polyclonal Antibody (A13097)

Western blot analysis of extracts of various cell lines, using SF3B6 antibody (A13097) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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